microbiology lab day 7 part 1 (day 7 bacterial growth curve, conjugation, Lederburg, Tatum, anaerobi

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mikepl103
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242720
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microbiology lab day 7 part 1 (day 7 bacterial growth curve, conjugation, Lederburg, Tatum, anaerobi
Updated:
2013-11-06 20:20:46
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microbiology lab day part bacterial growth curve conjugation Lederburg Tatum anaerobic API 20E system
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microbiology lab day 7 part 1 (day 7 bacterial growth curve, conjugation, Lederburg, Tatum, anaerobic growth, API 20E system)
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  1. each chamber in the API 20E system contains
    a microtube (the unopened part) and a cupule (the opened part)
  2. What is the API 20E System?
    API stands for Analytic Profile Index. It is a standardized, miniaturized version of conventional biochemical procedures used in the identification of Enterobacteriaceae (more than 5500 strains) and other gram negative bacteria ((more than 2300 strains)
  3. what are microorganisms that can only grow in oxygen called?
    obligate aerobes
  4. what are microorganisms that will grow either aerobically (grow faster) or in the absence of o2 (grow slower)?
    facultative anaerobes
  5. what are microorganisms that cannot use oxygen but aren't harmed by it, called?
    aerotolerant anaerobes
  6. Some microorganisms require only some oxygen. They are harmed by o2 at normal atmospheric tension. What are these called?
    microaerophiles
  7. Bacteria will grow in the part of the ___ ___ ___ that contains the proper O2 concentration
    agar deep culture
  8. What are two convenient ways to culture anaerobic bacteria
    cooked meat medium and thioglycollate broth
  9. What method can be used to culture bacteria that are anaerobes but aren't really fastidious anaerobes?
    Wright's tube. 
  10. Explain Wright's tube
    The anaerobic condition in Wright's tube is created using pyrogallol and NaOH. In the presence of NaOH, pyrogallol is oxidized and removes O2 very effectively in the process. After the anaerobic bacterium has been streaked out on the surface of the agar slant , a cotton plug is trimmed and then pushed into the culture tube until it rests just above the the top of the slant. The space between the top of the cotton plug anbd the open end of the culture tube is then filled with pyrogallol crystals, and 1 mL of 10% NaOH is added. the tube is closed iwth a rubber stopper and is immediately inverted. It is incubated upside down.
  11. What is the last step in the process of wright's tube?
    inverting the tube

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