microbiology lab day 7 part 2 (day 7 bacterial growth curve, conjugation, Lederburg, Tatum, anaerobi
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What is Brewer's anaerobic petri plate?
Brewer's special cover fits on a normal petri plate bottom in such a way that its circular ridge rests on the agar, thereby protecting most of the surface from exposure to O2.
What chemical does Brewer's anaerobic agar contain?
it contains a high concentration of thioglycollic acid.
what does the thioglycollic acid in brewer's anaerobic agar do?
the free sulfhydryl groups of the thioglycollate reduce any O2 present and create an anaerobic environment under the Brewer cover.
In the Gas Pak system, what does the GasPak envelope do?
hydrogen gas and co2 are generated by a gaspak envelope after the addition of water.
what does the palladium catalyst in the gaspak system do?
it catalyzes the formation of water from h2 and o2, thereby removing o2 from the sealed chamber.
how does the gas pak system work?
An envelope is added to a chamber. hydrogen and co2 are generated by a gaspak envelope after the addition of water. Once water is added to the envelope and it is put into the chamber, a lid is put over the chamber. Inside of the chamber is also a palladium catalyst which catalyzes the formation of H2O by combining H2 and O2.
the standard plate count provides information pertaining only to _____ bacteria
during the standard plate count, the plate should yield between ___ and ____ colonies
25 and 250
what is spectrophotometric analysis?
it is a way of analyzing the amount of bacteria in a medium. Light is passed through the medium and if there is a high population in the medium, then this means that less light is transmitted through. The light that is transmitted through is then converted to electrical energy and is measured by a galvonometer.
discovered by ____ and ______
Lederburg and Tatum
What is conjugation
- the transfer of DNA by direct cell-to-cell
- contact between bacterial cells
conjugation is a
mechanism of _____ ___ _____
horizontal gene transfer
during conjugation, a donor provides ____ or ___ ___ ___
conjugative or mobile genetic elements
what is an F factor?
- a conjugative plasmid that plays a major role in
- E coli
What would you like to do?
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