Chemiosmosis and ATP synthesis

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Author:
m_lievano
ID:
244756
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Chemiosmosis and ATP synthesis
Updated:
2013-11-03 17:41:29
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cytology
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Description:
unit 2
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  1. Electron transport chain Machinery:
    • Four enzymes in the mitochondrial inner membrane:
    • - NADH dehydrogenase
    • - Bc1 complex 
    • - Cytochome oxidase complex
    • - Succinate dehydrogenase 

    • Two electron carriers:
    • - Ubiquinone
    • -Cytochrome C
  2. Electron Transport Chain
    • -NADH n FADH2 need to b converted to ATP 
    • - happens in the inner membrane of mitochondria
    • - NADH and FADH2 bcome oxydized, allowing transporters to pump H+ across membrane.
    • - Generate electrical current (charge different across the membrane from H+ being pump out of mitochondria) 
    • - H+ flow through ATP synthase.
  3. Chemiosmosis
    • NADH n FADH2 both donate 2e- 
    • - NADH to Bc 1 complex
    • - FADH2 to NADH dehydrogenase 

    These high-energy e- re transfer from complexes w low e- affinity. (NADH dehydrogenase) to those w higher affinity (cytochrome oxidase complex)
  4. Chemiosmosis (part2)
    Reduction of complexes allows H+ to b pumped out of matrix to inner membrane space, creating a charge gradient and H+ gradient.

    Succinate dehydrogenase (las step of energy harvest II in krebs) reduces ubiquinone using FADH2 as an e- carrier
  5. ATP synthase
    - F0 is a rotor that is powered by H+. Rotation turns Fi through a stator.

    • - Fi has 3alpha n 3betas sub units.
    •   > sub units bind ADP and Pi force them together to make ATP (lose, tight, open comformation). 

    • - Fi creates ATP as it turns
    •   > 3.33 ATP per revolution or 10H
  6. Aerobic respiration
    • - Oxygen is the terminal e- acceptor 
    •   > produces water
  7. Energy from 1 molecule glucose ATP
    • - Glycolysis: 
    •   > 2 ATP  
    •   > 2 NADH x 2.5 = 1 ATP/molecule

    • - Krebs:
    •   > 6 NADH x 2.5 ATP
    •   > 2 FADH x 1.5 ATP(o.75+0.75 = 1.5) 
    •   > 2 GTP x 1 ATP   
    • * 30 ATP eukariote 
    • * 32 ATP prokariote

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