microbiology lab days 5 and 6 part 1 (biochemical assay, fermentation, beta galactosidase, onpg, TSI

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mikepl103
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244757
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microbiology lab days 5 and 6 part 1 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
Updated:
2013-11-03 18:10:22
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microbiology lab days part biochemical assay fermentation beta galactosidase onpg TSI imvic gelatin catalase coagulase dnase oxidase
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microbiology lab days 5 and 6 part 1 (biochemical assay, fermentation, beta galactosidase, onpg, TSI, imvic, gelatin, catalase, coagulase, dnase, oxidase)
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  1. what is fermentation?
    an energy producing biochemical reaction i nwhich organic molecules serve as both electron acceptors and electron donors.
  2. Phenol
    red broth is used to detect ________ in bacteria
    fermentation
  3. Phenol red is _____ below pH 6.8,_____ above
    7.4, and ____ between these two points.
    yellow, purple-pink, and red
  4. a ____ ___ is used to detect whether or not a bacterium produces gas
    durham tube
  5. how do you tell what the results are for a fermentation test?
    red color no gas - no fermentation

    yellow color no gas - acid production

    yelow color and gas - acid and gas production

    red color and gas - alcohol fermentation

  6. what does B-galactosidase do?
    it breaks down lactose to galactose and glucose
  7. what chemical is used in B-galactosidase detection?
    ONPG
  8. how does the B-galactosidase test work?
    ONPG is cleaved into o-nitrophenol by B-galac., which yields a yellow color.

  9. what is a key tablet used for?
    it can be used to detect B-galc. activity. If it turns yellow B-galac. is present
  10. TSI is used to identify and distinguish the _____ from other ___ ___ organisms
    enterobacteriaceae from other gram negative organisms
  11. What is contained in the agar in the Triple Sugar Iron Agar test?
    1% lactose and sucrose, 0.1% glucose, Phenol red
  12. acetoin is a precursor of
    2,3-butanediol
  13. barritt's reagent is used in what test?
    voges proskauer
  14. What type of agar is used in gelatin hydrolysis test?
    nutrient gelatin deep tubes
  15. what is a positive gelatin test?
    liquified medium

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