microbiology lab days 5 and 6 part 5 (biochemical assay, fermentation, beta galactosidase, onpg, TSI

Card Set Information

Author:
mikepl103
ID:
245560
Filename:
microbiology lab days 5 and 6 part 5 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
Updated:
2013-11-06 22:28:17
Tags:
microbiology lab days part biochemical assay fermentation beta galactosidase onpg TSI imvic gelatin catalase coagulase dnase oxidase
Folders:

Description:
microbiology lab days 5 and 6 part 5 (biochemical assay, fermentation, beta galactosidase, onpg, TSI, imvic, gelatin, catalase, coagulase, dnase, oxidase)
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user mikepl103 on FreezingBlue Flashcards. What would you like to do?


  1. what does superoxide dismutase do?
    it catalyzes the destruction of superoxide
  2. what do catalase and peroxidase do?
    catalyze the destruction of H2O2
  3. What is a postive oxidase test?
    change from pink color to dark purple
  4. What is a positive phenylalanine deaminase test?
    Green color
  5. What is phenylalanin deaminase?
    an enzyme tht catalyzes the removal of the amino group from phenylalanine.
  6. During the phenylalanine deaminase test, what is done to the medium once a bacterium has been incubated on it?
    ferric chloride is added
  7. what is a negative test for phenylalanine deaminase?
    no color change to green
  8. in the nitrate reduction test, if a non-fermenter yields gas and no red color, what does this mean?
    Nitrate was reduced to N2 only
  9. If the nitrate reduction test yields a red color after the addition of reagents A and B, what does this mean?
    Nitrate was reduced to nitrite
  10. in the nitrate reduction test, If zinc is added to the medium and the medium turns red, what does this mean?
    nitrate was not reduced
  11. in the nitrate reduction test, If one adds reagents A and B as well as zinc and no color change is observed, what does this mean?
    nitrate reduced to ammonia or hydroxylamine
  12. What is the principle of the nitrate reduction test?
    bacteria that are able to reduce nitrate can produce a variety of metabolites (ammonia, nitrite, nitrogen gas, etc.) while some bacteria aren't able to reduce nitrate. These distinctions allow us to differentiate many different strains of bacteria
  13. what molecules can nitrate be reduced to?
    nitrite, nitrogen gas, ammonia, hydroxylamine
  14. in the TSI agar test, if a red butt and red slant is observed, the bacterium cannot be from the ___________
    Enterobacteriaceae
  15. What is cysteine desulfurase?
    an enzyme that cleaves the sulfur atom from cysteine to form hydrogen sulfide gas

What would you like to do?

Home > Flashcards > Print Preview