microbiology lab days 5 and 6 part 6 (biochemical assay, fermentation, beta galactosidase, onpg, TSI

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mikepl103
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245569
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microbiology lab days 5 and 6 part 6 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
Updated:
2013-11-06 22:23:38
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microbiology lab days part biochemical assay fermentation beta galactosidase onpg TSI imvic gelatin catalase coagulase dnase oxidase
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microbiology lab days 5 and 6 part 6 (biochemical assay, fermentation, beta galactosidase, onpg, TSI, imvic, gelatin, catalase, coagulase, dnase, oxidase)
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  1. What are the components of the SIM medium?
    peptones and sodium thiosulfate as substrates, and ferrous ammonium sulfate as the H2S indicator
  2. What can the SIM medium be used to detect?
    it may be used to detect H2S production, motility, and indole production
  3. If a bacterium produces H2S in SIM medium, what will occur in the medium?
    Once H2S is produced ,it combines with the ferrous ammonium sulfate, forming an insoluble, black ferrous sulfide precipitate that can be seen along the line of the stab inoculation. If the organism is also motile, the entire tube may turn black
  4. How can one use the SIM medium to determine if a bacterium is motile?
    Motility is present when the growth of the culture is not restricted to the stab line of the inoculation.
  5. What chemical can be added to the medium to determine if the bacterium is motile?
    tetrazolium salt
  6. if terazolium salt is added to the medium, a positive test for motility is indicated by what?
    a red color radiating out from the stab
  7. if tetrazolium salt is added to the medium, a negative test for motility is indicated by what?
    a red color lining the inoculating stab
  8. The IMViC test may be used for the differentiation and identification of ____ bacteria
    enteric
  9. The IMViC test takes advantage of the fact that different enteric bacteria _____ ______ in different ways
    metabolize glucose

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