microbiology lab days 5 and 6 part 6 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
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What are the components of the SIM medium?
peptones and sodium thiosulfate as substrates, and ferrous ammonium sulfate as the H2S indicator
What can the SIM medium be used to detect?
it may be used to detect H2S production, motility, and indole production
If a bacterium produces H2S in SIM medium, what will occur in the medium?
Once H2S is produced ,it combines with the ferrous ammonium sulfate, forming an insoluble, black ferrous sulfide precipitate that can be seen along the line of the stab inoculation. If the organism is also motile, the entire tube may turn black
How can one use the SIM medium to determine if a bacterium is motile?
Motility is present when the growth of the culture is not restricted to the stab line of the inoculation.
What chemical can be added to the medium to determine if the bacterium is motile?
if terazolium salt is added to the medium, a positive test for motility is indicated by what?
a red color radiating out from the stab
if tetrazolium salt is added to the medium, a negative test for motility is indicated by what?
a red color lining the inoculating stab
The IMViC test may be used for the differentiation and identification of ____ bacteria
The IMViC test takes advantage of the fact that different enteric bacteria _____ ______ in different ways