Biochem 501: Lecture 30: Biosynthesis of AA and Porphyrins

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Biochem 501: Lecture 30: Biosynthesis of AA and Porphyrins
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2013-11-12 21:19:31
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Biochem 501
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  1. Where are all AA derived from?
    Intermediates in glycolysis, TCA cycle or pentose phosphate pathway
  2. How does nitrogen enter Glycolysis, TCA, and Pentose shunt?
    Via glutamine and glutamate
  3. How many AA can Bacteria/Plants/Animals synthesize
    • Plants/bacteria: All 20
    • Animals: Can only synthesize 10 simple AA, need to eat the rest (the ones eaten are the essential aa)
  4. Overview of AA Biosynthesis
  5. General Transamination Reaction
    • Carried out by aminotransferase
    • Switches O and NH3 f carboxylic acid and amine
  6. Role of alpha-keto glutarate
  7. Non-essential AA pathways
  8. 3-Phosphoglycerate Family (Ser, Gly, Cys)
  9. Arginine Synthesis
    • Comes from alpha-ketoglutarate
    • Via Urea cycle
    • Children need it, adults make enough
  10. Synthesis of Tyr, Phe, Trp
  11. All amino acids derived from what intermediates:
    Glucose, 3-Phosphoglyceric Acid, Phosphoenolpyruvic acid, Pyruvate, alpha-KG, OAA
  12. Essential Human Amino Acids
    • Val, Met, His, Leu, Phe, Lys, Ile, Thr, Trp, Arg
    • Very Many Hairy Little Pigs Live In The Torrid Argentina
  13. Porphyrin Structure
  14. Porphyrin Biosynthesis


    • Glycine is basic part
    • Aminolevulinate is used in repetitive condensation reactions to make porphyrin
  15. Heme B and Chlorophyll
    • Porphyrins:
    • Heme b has cofactor Iron, Chlorophyll has cofactor Mg
    • Chlorophyll also has fatty acid chain to help anchor it

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