BCHM 307 Quiz V

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Author:
MRK
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247098
Filename:
BCHM 307 Quiz V
Updated:
2013-11-18 14:02:22
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TCA oxphos
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  1. Citric Acid Cycle (general)
    • kreb cycle
    • TCA cycle
    • requires aerobic conditions
    • occurs in matrix of mitochondrion in eukaryotes
    • cytosol of prokaryotes
  2. Summary or citric acid equation
    • pyruvate + 4 NAD+ + FAD + GDP + Pi + 2 H2O ->
    • 3 CO2 + 4 NADH + 4H+ GTP + FADH2
  3. What is the Endosymbiont theory
    mitochonidia were once prokaryotic bacteria
  4. pyruvate dehydrogenase complex
    • links glycolysis to the citric acid cycle
    • happens in the matrix of mitochonida
    • Its a cluster of several enzymes
    • makes Acetyl-CoA
  5. Coenzyme A
    • carries the Acetyl from cytoplasm to mitochondia
    • has a free thiol group that can form thioesters
  6. NAD+ and NADH
    • NAD accepts H
    • NADH is used to make ATP 1:3
  7. dehydrogenase
    • enzyme used in redox rxn
    • usually has NAD+ reactant
  8. Succinate Dehydrogenase
    • associated with inner mitochondiral membrane
    • FAD to FADH2
  9. What energy currency does the citric acid cycle make
    • ATP/GTP
    • NADH
    • FADH2
  10. What is the purpose of the citric acid cycle
    • produces compounds to make ATP
    • as it goes around e- are pulled out (oxidizing)
  11. What is the purpose of the electron transport chain
    • capture energy in redox potential of NADH and FADH2
    • Used for ATP production
  12. What is Oxidation Phosphorylation
    using the protons generated in the e- transport chain to make ATP
  13. ATP synthase complex
    • F0 proton pore
    • F1 ATP synthesizing subunits
    • 1 ATP for every 3 protons
    • (NADH ~3ATP, FADH2 ~ 2ATP)
  14. NTPs NADH FADH NTP Oxphos NTP Total
    Glycolosis
    PDC
    TCA
    Total
    • 2  2  0     6     -
    • 0  2  0     6     -
    • 2  6  2  18+4  -
    • 4  -  -     34   38
  15. e- from NADH enter e- transport chain @
    complex I
  16. e- from FADH2 enter e- transport chain @
    Complex II
  17. what are the e- carriers in complex I
    • flavoprotein (FMN <-> FMNH2)
    • Fe-S (Fe3+ <-> Fe2+)
  18. What are the e- carriers in Complex II
    • flavoprotein (FMN <-> FMNH2)
    • Fe-S (Fe3+ <-> Fe2+)

     succinate dehydrogenase
  19. What are the e- carriers in Complex III
    • cytochromes (heme-containing proteins)
    • Fe-S (Fe3+ <-> Fe2+)
  20. Coenzymne Q (Ubiquinone or CoQ)
    • membrane soluble 
    • lite compound
    • shuttles e- & proton from I/II to III
    • has a long hydrophobic tail that keeps it in mitochondrial inner membrane
  21. Cytochrome C
    • contains Fe
    • can leave the mitochondria and trigger cell death
    • A peripheral membrane proteins
    • shuttles e- and protons from III to IV
  22. Carriers for Complex IV
    • 2 cytochromes
    • protien with Fe (fe3 to fe2)
    • protien with Cu (cu2 to cu1)
  23. what metal is most as e- carrier used and second
    Fe then Cu
  24. Electronchemical gradient
    • H on north side chemical
    • H+ = electrical
  25. when chain blocked
    • e- build up in chain
    • H20 not made
    • gradient can't be made
  26. when chain broken
    • electrons move through and water made
    • but gradient not formed
  27. NADH pumps ___ protons
    FADH2 pumps____ protons
    • 10
    • 6
  28. how many protons for ATP
    3 protons for each ATP

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