ASCP Micro

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  1. Anaerobic media Reducing agents
    (thio, cysteine) to remove O2
  2. CNA, PEA will only grow?

    • Colistin nalidixic acid in CNA;
    • Phenylethyl alcohol in PEA
  3. Blood Culture Collection
    • Prep skin with alcohol & iodine
    • Prior to fever spike
    • 2 – 3 cultures within 24 hrs
    • 1:5 – 1:10 blood to broth dilution
    • 10 mls for adults;
    • 1 – 5 mls for infants & kids
  4. Staph aureus resristances
    • Most resistant to penicillin (B-lactamase)
    • Most sensitive to penicillinase resistant penicillins (methicillin, oxacillin)
  5. Endocarditis; prosthetic device infections most often caused by?
  6. Aureus Biochem
    • Catalase positive
    • Coagulase neg vs pos
    • Growth in 7.5% NaCl
    • Ferment mannitol
  7. How to differentiate Epidermidis from saprophyticus ?
    • Novobiacin 
    • Sens  Staph epidermidis
    • Res  Staph saprophyticus
  8. Lancefield grouping for streptococcus is based on what?
    • C-mucopolysaccharide in cell membrane
    • Group A, B, D
  9. Beta hemolysis Strep are?
    • Strep pyogenes (B Strep Group A)
    • Strep agalactiae (B Strep Group B)
  10. Group A Strep Spec.?
    Pyogenes ( Beta Hemolytic)
  11. Group B Strep spec?
    • Agalactiae
    • Beta Hemolysis
  12. Alpha hemolytic Strep?
    • Strep pneumoniae
    • Enterococcus faecalis (Strep Group D)
    • Strep bovis (Group D, non-enterococcus)
    • Viridans Strep (alpha strep, not Group D)
  13. Strep Pneumo hemolysis?
  14. Group D Strep Spec?
    • Enterococcus Faecalis
    • Alpha
  15. Group D, non-enterococcus spec?
    • Strep Bovis
    • Alpha
  16. Alpha hemolytic Strep not group D Spec.?
    Viridans Strep
  17. Streptolysin stable in O2 Non antigenic
    • S
    • (Pyogenes)
  18. Streotolysin Oxygen labile; antigenic
    • O
    • (Pyogenes)
  19. Erythrogenic toxin in Pygenes causes?
    Scarlet Fever
  20. Can Cause Rheumatic fever and Acute glomerulonephritis
  21. Catalase: negative
    Bacitracin (A disk): positive
    Sensitive to Penicillin
    Resistant to SXT
  22. Neonatal sepsis
    Streptococcus agalactiae (Group B)
  23. Catalase: negative
    Bacitracin (A disk): neg
    CAMP rxn: positive
    Resistant to SXT
    Strep agalactiae
  24. Catalase: negative
    BEA: positive (esculin hydrolysis)
    6.5% NaCl: no growth
  25. Catalase: negative
    BEA: positive (esculin hydrolysis)
    6.5% NaCl: growth
    40% Bile: growth
    Enterococcus (Streptococcus) faecalis
  26. Lobar pneumonia
    Otitis media
  27. GP diplococci, lancet shaped
    concave colonies on BAP with alpha hemolysis;
  28. Catalase: negative
    Bile soluble
    Optochin (P disk): sensitive
    Strep pneumoniae
  29. Endocarditis Normal flora to oral cavity; bacteremia following dental procedures
    Viridans streptococcus
  30. STD, Venereal disease Gram stain sensitive for 99.9% of malesIf intracellular gram negative diplococci on GS, do not need to confirm with culture
    Neisseria gonorrhoeae
  31. GS: gram neg intracellular diplococciGrowth: fastitidious, growth on choc or MTM in CO2
    N. gonorrhoeae
  32. Oxidase: positive
    CTA sugars: ferments glucose
    Beta lactamase: perform to determine if P sensitive
    N. gonorrhoeae
  33. Transmitted by respiratory droplets (close contact)
    Meningitis in children < 3 yrs old
    Neisseria meningitidis
  34. Oxidase: positive
    CTA sugars: ferments glucose & maltose
    Beta lactamase: perform to determine if P sensitive
    Neisseria meningitidis
  35. Bacterial meningitis
    High neutrophil count in CSF
    Low glucose value in CSF
    High protein conc in CSF
    Neisseria meningitidis
  36. Transmitted by respiratory droplets (close contact)
    Respiratory infections
    Moraxella catarrhalis
  37. Oxidase: positive
    CTA sugars: neg for glu, mal, suc, lac
    Beta lactamase positive

    gram neg diplococci
    Moraxella catarrhalis
  38. Can be normal flora to vaginal tract
    Bacterial vaginosis
    Clue cells  vaginal epithelium covered in bacteria
    Gardnerella vaginalis
  39. GS: gram neg cocci
    Growth: grows on human BAP (V agar) and Choc in
    CO210% KOH  fishy smell
    Gardnerella vaginalis
  40. Conjunctivitis  Pink eye
    H. aegyptius
  41. Meningitis (1 month – 2 yrs)
    Epiglottitis (2 – 4 yrs)
    Haemophilus influenzae
  42. STD  soft chancre (chancroid)
    H. ducreyi
  43. X & V required
    Haemophilus influenzae
  44. X & V required (from eye culture)
    Haemophilus aegyptius
  45. X only
    Haemophilus ducreyi:
  46. GS: pleomorphic with clubbed ends;
    chinese letters;
    Methylene blue (Loeffler’s agar): metachromatic granules observed
    Corynebacterium diphtheriae
  47. Catalase positive
    Tinsdale agar: black colonies due to tellurite hydrolysis
    Elek test: determines toxins produced
    Corynebacterium diphtheriae
  48. gram pos rods
    Morph: small colonies with narrow zone of hemolysis
    Listeria monocytogenes
  49. Catalase: positive
    BEA: positive
    Motility: tumbling motility with hanging drop; umbrella motility @ RT, but not at 37 C
    Listeria monocytogenes
  50. Occupational infection for fishermen, butchers, rose growers
    GS: slender, gram pos rods
  51. Catalase negative
    Motility: non-motile
    TSI: H2S pos; can be confused with Salmonella because of H2S production
  52. Large gram pos rods; spore formers
    Large ground glass colonies with
    beta hemolysisCatalase positive
    Bacillus species
  53. GS: large
    GPR; spore former 
    bamboo shootsMorph:
    Medusa head colonies
    Bacillus anthracis
  54. Oxidase negative
    Glucose fermenters
    Peritrichous flagella when motile
  55. Indole pos
    Lactose fermenter
    MR pos/VP neg
    Citrate neg
    Enterobacteriaceae:  E coli
Card Set
ASCP Micro
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