MMI 302: Chemotaxis

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MMI 302: Chemotaxis
2013-11-19 01:46:22
MMI 302

MMI 302
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  1. How does a bacteria achieve flagella motion
    • -Synthesize a flagella
    • -Have a means to turn it
    • -Have a mechanism to direct the cell
  2. What happens when flagella go clockwise vrs. counter clockwise
    • Clockwise=fly apart
    • Counter-clockwise= work together
  3. Uses for flagella
    • TransitMechanosensing
    • Biofilm formation
    • Production of outer membrane vesicles
    • Flagellar type-3 secretion
    • Adhesion to host cells
    • Recognition by host receptors
  4. Example of flagellar transit
    Uropathogenic E. coli
  5. Example of mechanosensing
    Vibrio parahaemolyticus
  6. Example of biofilm production
    Listeria monocytogenes
  7. Example of production of outer membrane vesicles
    Vibrio fischeri/cholerae
  8. Example of flagellar type-3 secretion
    Yersinia enterolitica
  9. Adhesion to host cells
    Aeromonas spp.
  10. Example of recognition by host receptors
    Toll-like receptor 5
  11. How can flagella be a deciding factor in which bacteria enter bobtail squid
    • Mucus layer-->Keeps out gram +
    • Pores can only be entered by FLAGELLATE fischeri
  12. Structure of bacterial flagellum
    Basal body on membrane, motor proteins adjacent, hook and rod proteins protrude, flagellin monomers heteromerize outwards
  13. Purpose of basal body
    • Secrete flagellin subunits
    • Can also secrete proteins (type-3)
  14. How does cell use flagella to go towards/away from something
    • Attractant/Repellent proteins on outer membrane sense something they are attracted to or repelled from, and direct flagella on opposite side accordingly
    • (Series of Che signals)
  15. Rings
    • First outer ring of those searching for something not on plate
    • Inner ring is stuff that found it
    • Can disrupt ring by adding something they want.