Molecular Biology Exam 3

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Anonymous
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249184
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Molecular Biology Exam 3
Updated:
2013-11-26 10:55:40
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Molecular Test
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Molecular Test
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  1. Plasmids are usually linear.
    T or F
    False
  2. Plasmids are not found in eukaryotes.
    T or F
    False
  3. Many bacterial plasmids can replicate up to 20 times before cell division.
    T or F
    True
  4. Conjugative plasmids that can transfer to other bacteria through rolling circle replication like the F plasmid in E. coli tend to be single-copy plasmids.
    T or F
    True
  5. Linear plasmids that encode polymerases can be found in certain mitochondria.
    T or F
    True
  6. Plasmids can also encode toxins like colicins to kill other bacteria.
    T or F
    True
  7. Which statement about plasmids is false?
    None. All are true.
  8. What 2 things are essential for making a plasmid a vector? (1 answer only)
    A selectable marker and a multi-copy origin of replication.
  9. Which statement about antibiotic resistance is false?
    Chloramphenicol resistance involves the plasmid-born alteration of ribosomes or the chromosomally encoded CAT enzyme inactivating the chloramphenicol by acetylation.
  10. Which statement about PCR is true?
    Hot-start polymerases help prevent unwanted product and primer-dimers by becoming active only when the temperature is high enough.
  11. You are setting up a PCR reaction and have added template, buffer with MgCl2, dNTPs, and Taq polymerase, and possibly water to your PCR tube. Name the missing reagent(s) in the reaction:
    Forward and Reverse Primers
  12. Which statement about RT-PCR is false?
    It is a method for amplifying regions of genomic DNA.
  13. Which statement about RAPDs is false?
    It is a way to rapidly amplify cDNA ends from partial cDNA sequence.
  14. Which one of the below techniques can be used to remove proteins from your DNA preps?
    All will work.
  15. Which statement about DGGE is true?
    All are true.
  16. Mini-preps are used to isolate small, supercoiled plasmid DNA as well as chromosomal (genomic) DNA. 
    T or F
    False
  17. In the mini-prep procedure, cells are lysed under alkaline conditions, denaturing both nucleic acids and proteins.
    T or F
    True
  18. After the alkaline mini-prep mixture is neutralized by the addition of potassium acetate, chromosomal DNA and proteins precipitate because it is impossible for them to renature correctly. 
    T or F
    True
  19. Once the alkaline mini-prep mixture is neutralized, plasmids renature correctly and stay in solution.
    T or F
    True
  20. The OD 260/280 ratio from your spectrophotometer readings of your plasmid mini-prep shows the purity of your DNA prep.
    T or F
    True
  21. If the OD 260/280 of your mini-prep spec reading is >1.8 you’ve got protein and if it’s <1.8 you’ve got RNA, and if it's 1.8 it's just right.
    T or F
    False
  22. 1 OD260 Unit  = 50µg/ml for dsDNA from your mini-prep spec readings.
    T or F
    True
  23. Which statement about FISH is false?
    FISH can be used to look at DNA within tissue sections (that’s why the I and S stand for in situ) but not chromosome smears or squashes.
  24. In vitro mutagenesis involves...
    All of them
  25. Which statement about oligo-making is false?
    DMT blocks 3’ OH & phosphoramidite activates 5’ OH
  26. Which statement about PNA is false?
    PNAs are not degraded by nucleases but are degraded by proteases.
  27. TA cloning relies on Taq polymerase adding an A to the 3’ ends of the PCR amplicon for cloning into a linear vector containing Ts on its ends.
    T or F
    True
  28. The beauty of Taqman probes is that they increase specificity of your real-time PCR by acting as an extra primer.
    T or F
    False
  29. PFU DNA polymerase is now the enzyme of choice for TA cloning because of its proofreading ability.
    T or F
    False
  30. The beauty of Scorpion probes is that they increase specificity of your real-time PCR by combining the primer and beacon in one fragment of DNA.
    T or F
    True
  31. RACE is used to isolate either the 5’ or 3’ ends of partial cDNA clones.
    T or F
    True
  32. When performing a SYBR green real time PCR assay, the fluorescence is read during the extension stage.
    T or F
    True
  33. Primer tailing is a commonly used way to add extra sequence to your amplicon during PCR.  But keep in mind, that sequence included in your primer at the 5’ end will not be incorporated into the final PCR product.
    T or F
    False
  34. Cell lysis is aided by chemicals such as detergents or enzymes, but always needs an extra mechanical step like bead beating or sonication. 
    T or F
    False
  35. Molecular beacons...
    Fluoresce when bound to target DNA.
  36. Which statement about methylation specific PCR is true?
    It is used in eukaryotes for epigenetic studies

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