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How much bacteria in what location and for how long?
- Stomach 103 about 15-20 min
- Small intestine 106 about 2-3 hours
- Colon 1012 about 24-72 hours
What are the 5 etiological agent that are likely to cause toxin actions in the GI tract?
- Clostridium batulinum
- Staph aureus (food poisoning)
- Bacilus cereus
- Clostridium difficile
- Enterotoxigenic E. coli (ETEC)
Action of toxins table 14-3
What etiological agent causes gastritis?
What kind of bacteria is helicobacter pylori?
- gm - spirillum
- or bacillary form w/ sheathed flagella
What are the lab test of helicobacter pylori?
Catalase, urease + and oxidase +
helicobacter pylori what kind of other test?
- dont ferment or oxidize carbohydrates
- DIFFICULT to culture
How does bacteria live in acid stomach?
How does it enter? secretes?
- penetrates mucus to epithelium
- endotoxin--> inflammatory response
What of helicobacter pylori has low activity?
Lipid A low endotoxin activity
helicobacter pylori causes what %?
- 80% of gastric ulcers
- 90% dueodenal ulcers
Lower GI diseases such as what 3?
- Pysentery-diarrhea w/ pus/blood in stool
- Enteric fever- high fever & abscesses
What kind of bacteria is vibrio? How many chromosomes?
- gm - mesophillic, curved rods
- 2 chromosomes
Where is vibrio found? degrades what?
- estuarine and marine waters
What kind of host does vibrio have?
humans, oysters, clams, fish, mussels, plankton, shellfish
How many biotypes are there of V. vulnificus?
What does biotype 1 do?
- opportunistic human pathogen
- gastroenteritis, primary septicemia & wound infections (necrotizing fascitis)
- Human wound infections
- fisherman handling tilapia
Vibrio Cholera, found where, prod what?
- rice water stools
- killed by acidic conditions
- cont water w/ human feces
- A-B exotoxins
What is the action of Cholera toxins?
- B subunit binds to host cell surface
- A subunit enters cell