lab quiz 4

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nando54321
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250065
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lab quiz 4
Updated:
2013-12-01 21:36:17
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micriobiology 41
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Weeks 8-10
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  1. what is the function of EMB agar?
    to identify fermentation of various sugars
  2. what are the two sugars used in EMB agar?
    lactose and sucrose
  3. what does fermentation of sugars do in an EMB agar?
    1-sugars encourage the growth of enteric bacteria

    2-it differentiates them based on color reaction created when combine the dyes
  4. what type of coliform does lactose support in EMB agar?
    supports the growth of  Escherichia Coli (E.coli)
  5. what type of coliforms does sucrose support in EMB agar?
    pathogens such as Proteus or Salmonella
  6. what is the purpose of the Eosin and methylene blue dyes in EMB agar?
    1-Inhibit the the growth of of gram positive organisms

    2-react vigorously with lactose fermenters and turn the growth dark purple or black
  7. the green metallic sheen in EMB is typical of what?
    the presence of E.Coli
  8. what colors do less aggressive lactose fermenters produce in EMB?
    pink to dark purple
  9. what is EMB agar selective for?
    encourages growth of Gram(-) and inhibits G(+)
  10. What is the purpose of phenol red broth?
    to demonstrate acid and gas production of organism
  11. what causes gas production in PR broth?
    fermentation of carbohydrate in the tube
  12. what does yellow broth, bubble in tube mean in Phenol Red Broth?
    fermentation and acid production A/G
  13. what does yellow broth no bubble in Phenol red broth mean?
    fermentation but no gas A/-
  14. what does red broth, no bubble in tube mean in the phenol red broth test?
    no fermentation, no gas production -/-
  15. what does pink broth, no bubble mean in the phenol red broth?
    degradation of peptone;alkaline end products
  16. what causes phenol red broth to turn pink?
    deamination of peptone amino acids producing Ammonia
  17. what is Simmons Citrate agar used for?
    to see if organism can survive on sole carbon and nitrogen source
  18. In the simmons citrate agar what is used for sole carbon source?
    sodium citrate
  19. in the simmons citrate agar what is used as the sole source of nitrogen?
    ammonium phosphate
  20. what color is bromthymol blue dye at 6.9ph?
    green
  21. what color is the bronthymol dye at 7.6ph?
    blue
  22. what does blue in citrate test mean?
    citrate is utilized
  23. what does no color change but organism growth on the citrate test mean?
    citrate is utilized
  24. what does no color change (stays green) and no growth of organism mean?
    citrate is not utilized
  25. what is the SIM test used for?
    • to determine
    • 1-sulfur reduction
    • 2-indole production from tryptophan
    • 3-motility
  26. what does doe the color black in the SIM media mean?
    sulfur reduction and a positive test
  27. what does a layer of red mean in the SIM test?
    it indicates positive for indole and tryptophanase presense
  28. what effect does UV light C have on bacteria?
    its detrimental, expose to UV-C causes irreparable DNA damage to organism
  29. Leukocytes are two divided into which two groups?
    • 1-granulocytes
    • 2-agranulocytes
  30. what are the different types of granulocytes?
    • 1-neutrophils
    • 2-basophils
    • 3-eosinophils
  31. what is a typical trait in a neurophil?
    segmented nucleus
  32. what percent of neutrophils make up WBC?
    55-65%
  33. what is a typical trait of basophil?
    dark purple cytoplasmic granules that obscure the nucleus
  34. what percent of basophils make up the total number of WBC?
    .05-1%
  35. what is a typical trait of eosinophils?
    2x as big as RBC and contain two lobes that tends to make them look like aliens
  36. what percent of Eosinophils make up the total number of WBC?
    1-3%
  37. how many types of agranulocytes are there?
    • two
    • 1-monocytes
    • 2-lymphocytes
  38. what is a typical trait of a monocyte?
    a round large indented nucleus
  39. what percent of monocytes make up the total number of WBC
    3-7%
  40. what is a typical trait of a lymphocyte?
    large nuclues with a thin halo of cytoplasm encircling their nucleus
  41. what is the percent of lymphocyte that make up the total number of WBC?
    25-33%
  42. what are the WBC cells of the immune system?
    lymphocytes
  43. what are the functional types of lymphocytes?
    • 1-T-cells
    • 2-B-cells
    • 3-larger natural killer cells
  44. what group and type of cell is this?
    granulocyte and its a basophil
  45. what group and type of cell is this?
    Granulocyte and its a eosinophil
  46. what group and type of cell is this?
    Agranuloyte and its a lymphocyte
  47. what group and type of cell is this?
    Agranulocyte and its a monnocyte
  48. what group and type of cell is this?
    granulocyte and its a neutrophil

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