Biochem 501: Part IV.1: Genetic Transfer

The flashcards below were created by user Anonymous on FreezingBlue Flashcards.

  1. Main components of genetic information transfer
    Amino acids and Nucleotides
  2. Key reaction in formation of Nucleoprotein complex:
    Phosphoryl transfer
  3. Griffith's Experiment
    • Living S cell (Fatal)
    • Living R cells (Non-fatal)
    • Heat-killed S cells (Non-fatal)
    • Heat-killed S cells and living R cells (Fatal)
    •      -Living S cells found in blood sample
    •      -Fatal nature of S cells survived heat-killing and transferred to R cells.
  4. Avery Experiment:
    • Took RNA, Protein, DNA, lipids, and carbohydrates from S cells, added to R cells. Only the DNA cells became S cells.
    • DNA is genetic material
  5. Hershey-Chase:
    • Took a bacteriophage, marked both protein coat and DNA.
    • Observed infected bacteria
    • Tracers from DNA, not protein, observed in bacteria
    • DNA is infection vector for bacteria.
  6. Two types of nucleic acids:
    • Ribonucleic (have -OH at 2' posistion, RNA)
    • deoxyRibonucleic (have -H at 2', DNA)
  7. What are the Nucleosides:
    • Deoxyribonucleotides:
    • -Deoxyadenylate (dAMP)
    • -Deoxyguanylate (dGMP)
    • -Deoxythymidylate (dTMP)
    • -Deoxycytidylate (dCMP)
    • Ribonucleotides:
    • -Adenylate (AMP)
    • -Guanylate (GMP)
    • -Uridylate (UMP)
    • -Cytidylate (CMP)
  8. Chemical Components of a Nucleotide:
    Phosphate, Pentose, Purine/Pyrimidine base
  9. Nucleotides bases are what:
  10. What kind of bond joins the nitrogenous base to the pentose?
  11. Ribose adopts what forms in nucleotides
    beta-Furanose rings
  12. 'Sugar Pucker" of Furanose rings
    • One of the carbons or the oxygen is not in line, and can be 'in/endo' or 'out/exo' relative to the C5 chain that sticks out of the ring
    • Image Upload
    • This can effect distance between phosphates when nucleotides bind together and ultimately can effect the shape of the helix
  13. What type of bond links nucleic acids/how are they made
    • Phosphodiester
    • (Involves a Phosphate and creates two esters between the P and the two nucleotides, thus phosphodiester)
    • Phosphoryl transfer (transfer PPi for next nucleotide)
  14. How many strands can a nucleic acid chain have?
    1 or 2
  15. Base pairing
    • Bases pair with specific other bases when making double stranded
    • A-T (2 bonds) and C-G (3 bonds)
    • Hydrogen bonds
  16. Base stacking
    Bases have a specific distance between each other from Van der Waals forces, critical for stability
  17. Nucleotide have a what confomation relative to pentose?
    ANTI- Image Upload
  18. Two types of base-base bonding formations
    • Watson-Crick (anti-anti)
    • Image Upload
    • Image Upload
    • Hoogsteen (syn anti)
    • Image Upload
    • Image Upload
    • Image Upload
  19. Hoogsteen base pairing enables what?
    3 or 4 strands in a helix
  20. What furanose ring conformation is standard?
    C2' endo, B-DNA, W-C Double Helix
  21. B-DNA conformation allows proteins to do what?
    Contact nucleic acid bases
  22. Key differences between RNA and DNA
    • Suger is ribose, not deoxyribes
    • RNA is TYPICALLY single stranded
    • RNA is multi-functional: protein synthesis, gene expression, enzyme
  23. SS RNA does what?
    • Assumes varied complex secondary structures:
    • 'Hairpins'
Card Set:
Biochem 501: Part IV.1: Genetic Transfer
2013-12-06 00:00:20
Biochem 501 FINAL

Biochem 501 FINAL
Show Answers: