Of the three early models proposed for DNA replication, in which one was newly synthesized DNA incorporated into both template strands?
C. dispersive
In differental centrifugation:
C. rpm is varied, run time is kept constant
antibodies can be purified using a sucrose gradient centrifuged at:
B. 20,000 rpm at 5 hr
during DNA replication, new nucleotides are always added at:
C. the 3' hydroxyl of the preceding base
during lagging strand synthesis:
D. many RNA primers are made
Okazaki fragments are:
A. short stretches of DNA synthesized on the lagging strand
Proofreading of DNA synthesis takes place:
a. in the 3' -> 5' direction
b. in the 5' -> 3' direction
a. in the 3' -> 5' direction
proofreading of DNA synthesis is done by an enzyme other than DNA polymerase:
a. true
b. false
b. false
the enzyme telomerase:
C. (b and c)
Look at question 10-14 of exam I
Review the figure of DNA replication fork
What is a common sequence for a transcription promoter:
B. TATA
post-transcriptional modifications to eucaryotic mRNA include:
D. all of the above
the codon for initation of mRNA translation is:
A. AUG
Amino acids coded for by only one codon include:
A. tryptophan
the point mutation responsible for sickle cell anemia induces the following amino acid change in the b subunit of hemoglobin.
C. glu -> val
the fully assembled ribosome contains three sites important in protein synthesis. one of these is the P site, which stands for:
D. peptidyl-tRNA
in procaryotes, the first amino acid in newly synthesized protein in always:
a. n-formyl-methionine
b. methionine
a. n-formyl-methionine
during the termination sequence of protein synthesis, _________ binds to the ribosome at the A site:
A. release factor
control mechanisms of protein synthesis include:
D. all of the above
positive control of transcription in procaryotes can employ:
B. (a and c)
transcription regulators in drosophila include:
C. giant
a reporter gene is used to:
A. determine gene expression and d. create banded embryos
steroid hormones:
C. act within the nucleus
one way of controlling gene expression involves methylation of:
A. cytosine
mutations in somatic cells are inherited through the germ line:
a. true
b. false
b. false
types of mutations can include:
E. (a and c)
the ames test for chemical mutagens:
a. measures lactose tolerance
b. measures the back mutation His- ->His+ c. utilizes an E. coli auxotroph
d. measures the foward mutation His+ -> His-
b. measures the back mutation His-> His+
gene duplication occurs through:
D. recombination
exon shuffling:
A. involves transposon relocation
in procaryotes, the sex pilus is coded for by a ___________.
C. plasmid
Mobile genetic elements comprise approximately ________% of the human genome:
C. 50%
genetic differences between individuals can be determined by analyzing:
C. SNPs
stop codon sequences include:
E. (a, b and c)
DNA binding motifs include:
D. (b and c)
cancer can be the result of mutations induced by:
C. (a and c)
procaryotic genes have exons and introns:
a. true
b. false
b. false
the transcription factor TFIID:
D. (a and c)
the transcription factor TFIIH:
B. phosphorylates RNA polymerase II
only mature mRNA contain the following feature(s) can be exported from the nucleus:
D. (a and c)
amino acids bind to tRNA through a(n) _______ bond:
B. aminoacyl
during protein synthesis, the small subunit of the ribosome:
B. (a and c)
Molecular chaperones:
C. fold proteins into their 3D shape
post-transcriptional controls on protein synthesis include:
D. all of the above
initiator tRNA binds to the ______ site on ribosomes:
C. peptidyl-tRNA
in eucaryotes, RNA polymerase I is responsible for synthesis of:
