cell bio exam III key

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cell bio exam III key
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2013-12-07 02:21:34
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cell bio davis exam III
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  1. the following DNA nuclease produces straight, blunt ended cuts:
    A. HindIII
    B. EcoRI
    C. NotI
    D. HaeIII
    D. HaeIII
    (this multiple choice question has been scrambled)
  2. dissociation of dsDNA into single strands:
    A. requires a low pH
    B. (b or c)
    C. requires a high pH
    D. (a or b)
    E. requires high temperatures
    B. (b or c)
    (this multiple choice question has been scrambled)
  3. DNA from different individuals varies in:
    A. (a and b)
    B. (a and c)
    C. STRs
    D. rRNAs
    E. RFLPs
    B. (a and c)
    (this multiple choice question has been scrambled)
  4. leaky mitochondrial membranes:
    A. can be easily repaired
    B. are necessary for oxidative phosphorylation
    C. result in cell death
    C. result in cell death
    (this multiple choice question has been scrambled)
  5. cell membranes from the following organelles are exposed to the external environment:
    a. mitochondria
    b. golgi apparatus
    c. peroxisomes
    d. none of the above
    d. none of the above
  6. phosphatidylcholine contains:
    A. a carboxyl ion
    B. no phosphodiesters
    C. only saturated fatty acids
    D. a quaternary ammonium ion
    D. a quaternary ammonium ion
    (this multiple choice question has been scrambled)
  7. phosphatidylserine contains:
    A. no phosphodiesters
    B. terminal amino acid
    C. an ammonium ion
    D. a steroid group
    B. terminal amino acid and b. an ammonium ion
    (this multiple choice question has been scrambled)
  8. K+ ion concentrations:
    A. are 30 times higher outside the cell than inside
    B. are 10 times higher inside the cell than outside
    C. are 20 times higher outside the cell than inside
    D. are 30 times higher inside the cell than outside
    D. are 30 times higher inside the cell than outside
    (this multiple choice question has been scrambled)
  9. the passive transporter for glucose:
    A. works with the electrochemical gradient
    B. works with the concentration gradient
    C. works against the membrane potential
    D. works against the concentration gradient
    B. works with the concentration gradient
    (this multiple choice question has been scrambled)
  10. the maximum resting membrane potential for an animal cell is approximately:
    A. -2V
    B. -0.2 V
    C. +0.2 V
    D. -0.02 V
    B. -0.2 V
    (this multiple choice question has been scrambled)
  11. the following dye is used to visualize DNA bands after separation on agarose gels.
    A. indocyanine green
    B. ethidium bromide
    C. neutral red
    D. coumassie blue
    B. ethidium bromide
    (this multiple choice question has been scrambled)
  12. the polymerase chain reaction requires:
    a. a thermal cycler
    b. dNTPs
    c. Taq polymerase
    d. all of the above
    d. all of the above
  13. after 3 cycles of PCR, the number of dsDNA strands resulting from one initial dsDNA strand is:
    A. 16
    B. 4
    C. 8
    D. 2
    C. 8
    (this multiple choice question has been scrambled)
  14. reagents necessary to produce a recombinant DNA construct for use in E. coli include:
    a. a dsDNA fragment insert
    b. a dsDNA plasmid
    c. a restriction nuclease
    d. DNA ligase
    e. all of the above
    e. all of the above
  15. from what is cDNA made?
    A. mRNA
    B. dsRNA
    C. genomic ssDNA
    d. none of the above
    A. mRNA
    (this multiple choice question has been scrambled)
  16. the restriction nuclease HindIII produces:
    a. straight blunt ended cuts
    b. staggered cuts with "sticky ends"
    b. staggered cuts with "sticky" ends
  17. DNA can be quantitated by measuring absorbance at _______nm:
    A. 490 
    B. 260
    C. 470
    D. 280
    B. 260
    (this multiple choice question has been scrambled)
  18. Taq polymerase is obtained from the organism:
    A. bacillus thuringiensis
    B. thermus aquaticus
    C. bacillus thermoaquaticus
    d. none of the above
    B. thermus aquaticus and c. bacillus thermoaquaticus
    (this multiple choice question has been scrambled)
  19. eucaryotic systems for recombinant gene expression include:
    A. escherichia coli
    B. (a and b)
    C. pichia pastoris
    D. sacchromyces cerevisiae
    B. (a and b)
    (this multiple choice question has been scrambled)
  20. patterns of gene expression in different cell types can be determined by:
    A. replacing genes of interest with reporter genes
    B. sequencing genomic DNA
    C. sequencing rRNAs
    A. replacing genes of interest with reporter genes
    (this multiple choice question has been scrambled)
  21. genetic manipulations include:
    a. gene replacement with a mutant gene
    b. gene knockout
    c. gene addition
    d. all of the above
    d. all of the above
  22. a convenient source of natural membranes for experimentation is:
    A. leukocytes
    B. red blood cells
    C. brain tissue
    B. red blood cells
    (this multiple choice question has been scrambled)
  23. membrane scaffolding proteins include:
    A. actin
    B. (b and c)
    C. (a and c)
    D. spectrin
    E. myelin
    B. (b and c)
    (this multiple choice question has been scrambled)
  24. integral membrane proteins can be extracted with:
    A. triton x-100
    B. ethanol
    C. SDS
    D. (a and c)
    D. (a and c)
    (this multiple choice question has been scrambled)
  25. bacterial rhodopsin uses _________ to absorb light energy.
    A. retinal
    B. heme
    C. cytochromes
    D. chlorophyll
    A. retinal
    (this multiple choice question has been scrambled)
  26. cells can complex glycolipids:
    A. as energy sources
    B. as recognition motifs
    C. (b and c)
    D. in the inner membrane leaflet
    B. as recognition motifs
    (this multiple choice question has been scrambled)
  27. Concanavalin A is a(n):
    A. transporter
    B. structural protein
    C. enzyme
    D. lectin
    D. lectin
    (this multiple choice question has been scrambled)
  28. Membrane channels are selectively permeable due to:
    A. internal diameters
    B. specific amino acids lining the lumen
    C. (a and b)
    C. (a and b)
    (this multiple choice question has been scrambled)
  29. Ions with hydration shells can be transported by channels:
    a. true
    b. false
    b. false
  30. voltage gated Ca2+ channels:
    A. maintain resting membrane potential
    B. stimulate neurotransmitter release
    C. inhibit synaptic signaling
    B. stimulate neurotransmitter release
    (this multiple choice question has been scrambled)
  31. membrane channel control mechanisms include:
    A. ligand gating
    B. (a and b)
    C. stress gating
    B. (a and b)
    (this multiple choice question has been scrambled)
  32. The nernst equation for membrane potenial is:
    A. V = 62 X log10(co/ci)
    B. V = IR
    C. V = 62 X log10(ci/co)
    D. V = (Ci/Co)
    A. V = 62 X log10(co/ci)
    (this multiple choice question has been scrambled)
  33. the resting membrane potential is dependent primarily on _______ on both sides of the membrane. 
    A. [Na+]
    B. [H+]
    C. [Ca2+]
    D. [K+]
    D. [K+]
    (this multiple choice question has been scrambled)
  34. the primary advantage of cDNA is:
    A. lack of introns
    B. more active promoters
    C. regulatory sequences
    d. none of the above
    A. lack of introns
    (this multiple choice question has been scrambled)
  35. restriction mapping of genomal segments:
    A. is done with single nuclease
    B. is done with multiple nucleases
    C. generates overlapping sequences
    D. (b and c)
    D. (b and c)
    (this multiple choice question has been scrambled)
  36. Microarrays:
    A. use fluorescent cDNA probes
    B. use radioactively labeled cDNA probes
    C. (b and d)
    D. are prepared by hand
    E. require computers to use
    C. (b and d)
    (this multiple choice question has been scrambled)
  37. site directed mutagenesis requires restriction nucleases:
    a. true
    b. false
    b. false
  38. the glucose transporter:
    A. works with the concentration gradient
    B. (a and b)
    C. (a and c)
    D. transports D-glucose
    E. transports L-glucose
    C. (a and c)
    (this multiple choice question has been scrambled)
  39. the action potential in neurons is generated by:
    A. Na+ channels
    B. K+ channels
    C. H+ channels
    D. (a and b)
    A. (Na+ channels)
    (this multiple choice question has been scrambled)
  40. the Na+/K+ pump:
    A. transports Na+ out of the cell, K+ into the cell
    B. is dependent on ATP
    C. (a and b)
    D. (a and c)
    E. transports K+ out of the cell, Na+ out of the cell
    C. (a and b)
    (this multiple choice question has been scrambled)
  41. animal cells expend approximately _______% of their total ATP to transport Na+:
    A. 40%
    B. 10%
    C. 30%
    d. none of the above
    C. 30%
    (this multiple choice question has been scrambled)
  42. coupled membrane transport:
    A. can exchange one ligand for another across the membrane
    B. (a and b)
    C. can pump two ligands into the cell simultaneously
    d. none of the above
    B. (a and b)
    (this multiple choice question has been scrambled)
  43. the active glucose transporter:
    A. pumps glucose with the concentration gradient
    B. (a and b)
    C. (b and c)
    D. pumps glucose against the concentration gradient
    E. uses a K+ electrochemical gradient
    D. pumps glucose against the concnetration gradient
    (this multiple choice question has been scrambled)
  44. In intestinal epithelial cells:
    A. glucose is pumped in by active transport and out by passive transport
    B. glucose if pumped into and out of the cell by active transport
    C. glucose is pumped in by passive transport and pumped out by active transport
    A. glucose is pumped in by active transport and out by passive transport
    (this multiple choice question has been scrambled)
  45. plant cells modulate their ionic balance:
    A. by pumping H+ out of the cell
    B. (b and c)
    C. by pumping Na+ out of the cell
    D. by pumping K+ out of the cell
    A. by pumping H+ out of the cell
    (this multiple choice question has been scrambled)
  46. auditory hair cell sterocilia transform sound waves into an electrical signal with this ion channel:
    A. Na+
    B. K+
    C. Ca++
    D. H+
    C. Ca++
    (this multiple choice question has been scrambled)
  47. the action potential in neurons has a maximum amplitude of about:
    A. - 40 mV
    B. +60 mV
    C. +40 mV
    D. -60 mV
    C. +40 mV
    (this multiple choice question has been scrambled)
  48. the toxin ouabain:
    A. blocks signal delivery at neuromuscular junctions
    B. blocks the voltage gated calcium channel
    C. blocks inhibitory signals
    D. inhibits K+ binding to the Na+/K+ pump
    D. inhibits K+ binding to the Na+/K+ pump
    (this multiple choice question has been scrambled)
  49. neurotransmitters include:
    a. glutamate
    b. serotonin
    c. glycine
    d. (a and b)
    e. all of the above
    e. all of the above
  50. the toxin curare:
    A. causes muscle spasms and convulsions
    B. blocks inhibitory signals
    C. (a and b)
    D. causes muscle paralysis
    D. causes muscle paralysis
    (this multiple choice question has been scrambled)

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