Clinically significant yeast

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Clinically significant yeast
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2013-12-08 16:43:45
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Clinically significant yeast
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  1. What is endogenous in reference to yeast infections?
    It is the persons own normal flora withing body that is causing the infection.

    Most yeast infections are often endogenous**
  2. C. albicans

    Micro
    Macro
    Germ tube
    Sucrose
    Macro: Creamy off white

    Micro: Clusters of blastoconidia along pseudohyphae with terminal chlaymdoconidia

    Germ tube = POSITIVE

    Sucrose = POSITIVE

    *All the same for C. stellatoidea except--- Sucrose = Negative
  3. C. tropicalis

    Micro
    Macro
    Misc. info
    • Macro:
    • Creamy, glabrous with mycelial fringe

    • Micro:
    • Blastoconidia are single or small random clusters along pseudohyphae

    *Vaginitis, intestinal disease, systemic, & meningitis
  4. C. parapsilosis

    Macro
    Micro
    Disease
    • Macro: 
    • Creamy & glabrous

    • Micro:
    • Short crooked or curved pseudohyphae

    • Major cause of nosocomial infections
    • *indwelling catheter
  5. C. kreusi *Rare

    Macro
    Micro
    Disease
    • Macro:
    • Creamy & Flat

    • Micro:
    • Pseudohyphae & elongated blastoconidia branched like trees
  6. C. kefyr

    Macro
    Micro
    Disease
    • Macro:
    • Creamy & smooth

    • Micro:
    • Logs in a strem

    Common cause of UTI or endocarditis
  7. C. guilliermondii

    Macro
    Micro
    Disease
    • Macro:
    • Creamy flat, Glossy

    • Micro:
    • Short pseudohyphae with group of blastoconidia at constrictions

    UTI & endocartitis
  8. Torulopsis glabrata aka C. glabrata

    Macro
    Micro
    Disease
    • Macro:
    • Creamy, smooth, & moist

    • Micro:
    • Blastoconidia ONLY (on CMT), NO pseudohyphae or True hyphae

    UTI, endocarditis, meningitis
  9. Saccharomyces cerevisiae *working yeast

    Bread, beer, wine

    Macro
    Micro
    Occasionally normal flora

    • Macro: creamy, smooth, moist
    • Micro: Yeast cells and short pseudohyphae
  10. Cryptococcus spp.

    Disease
    Meningitis & pulmonary disease

    • C. neoformans
    • *Major cause on AIDS pt's
    • Found in soil & pigeon excreta
    • Meningitis is predilection for CNS
  11. Cryptococcus spp. Lab info

    *Unless specifed - I am referring to C. neoforman lab results
    • Macro:
    • Mucoid
    • on caffeic acid or bird seed agar - Melanin produced  (dark colony)

    • Micro:
    • DO NOT produce true or pseudohyphae on cornmeal agar. Blastoconidia only

    • India Ink detects capsule -Low detection rate
    • *All species are surrounded by capsule

    • Urease = POSITIVE
    • Nitrate = V
    • Phenol oxidase = Positive
    • (other cryptococcus spp are neg for phenol)


    Sugar assimilation varies
  12. Geotrichum candidum

    Macro
    Micro
    Disease
    • Macro: 
    • White, moist, yeast like
    • Micro:
    • True hyphae, arthroconidia *NO blastoconidia

    Rare intestinal tract infection
  13. Trichosporon beigelii (White piedra)

    Disease
    Micro
    • Emerging agent of disseminated infectin
    • *resistant to Amphotericin B

    Micro: Arthro & Blastconidia on cornmeal agar
  14. Malassezia furfur (Tinea versicolor)

    Disease
    Macro
    Micro
    Catheter related inf. in pt's in long term IV lipids

    • Macro: Creamy/brown wrinkled
    • Micro: Yeast like cells
  15. Sporobolomycess spp.


    Macro
    Micro
    • Macro:
    • Salmon colored smooth colonies
    • Micro:
    • Oval, elongated yeast cells with projectile spores

    *shoot from one plate to another
  16. Pneumocystitis jiroveci

    Disease
    Lab info
    Opportunistic cellular immunity

    *Fever, nonproductive cough, SOB, destroys alveolar cells

    Must demonstrate organism in tissue, lavage, or sputum

    cannot culture

    • GMS common - Deflated ball appearance
    • FTA available

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