Hepatitis

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Author:
ucstudent01
ID:
252381
Filename:
Hepatitis
Updated:
2013-12-10 16:50:54
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Hepatitis
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Hepatitis
Description:
Hepatitis
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  1. Hep A:

    Why is serology testing important
    To distinguish between immunity and acute infections.

    IgM present in acute phase

    Presence of IgG (which appears early in recovery phase) = immunity
  2. What tests are used for HEP A
    Indirect enzyme immunoassays

    or

    Direct competitive
  3. Vaccine stability and requirements for Hep A
    Recommended

    • lasts 14-20 years in children
    • 25 in adults
  4. What is present in the:

    Acute phase of Hep B
    1st top appear is: HbsAg

    followed closely behind

    HBeAg
  5. What is present in:
    Accute to early recovery of Hep B
    Anti-HBcAg

    with conversion of IgM to IgG
  6. What is present in:

    Immunity and recovery
    Anti-HBsAg

    early recovery: Anti-HBe+
  7. What is a sign of chronic infection with Hep B
    HBsAG and HBcAg

    *HBcAb can remain elevated for years
  8. What lab tests are utilized to identify Hep B
    EIA for Antigens

    Capture and Direct competitive assays for IgM Ab

    *Nationally reportable
  9. Hep D important info
    Can only have Hep D if you have Hep B.

    >prognosis for those with Hep B if also have Hep D
  10. What is the hallmark sign of progression of Hep C
    Mixed essential cryoglobulinemia
  11. What immunological assays are used for initial screening for Hep C
    EIA 3rd generation- Measures anti-HCV.

    Positive is if patient has Ab to any Ag's of Hep C.
  12. What is the Cut off value for Hep C
    It is the point at which a patient is considered reactive or positive.
  13. What is the confirmatory test for Hep C
    Molecular assays - 

    RIBA & NAT
  14. Principle of RIBA
    • Manufactured protiens on membrane bind with Ab in patient serum.
    • Indirect enzyme immunoassay done on strip. 
    • Enzyme labeled secondary to ab binds to pt ab

    *Test for viral RNA

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