Micro Biology

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Micro Biology
2014-01-21 12:44:56
Lecture 1 Vocabulary
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  1. What are the classification of Micro Organisms?
    • Monera
    • Prostista
    • Fungi
    • Plantae
    • Animalia
  2. What are the 3 domains of life of the Woese Diagram?
    • Bacteria
    • Archea
    • Eucarya
  3. What are Animalcules?
    Older term for microscopic animals
  4. What is Spontaneous Generation?
    • Its the Phenomenon where 'living organisms [can] develop from nonliving matter'
    •  ex: meat can spontaneously produce maggots.
  5. What did John Needham Discovered on Spontaneous Generation? (1748)
    • Heated meat broth, then stoppered
    • Became cloudy 
    • Orgamic matter contains vital force (turning nonliving into living)
  6. What did Lazzaro Spallanzani Discovered on Spontaneous Generation?
    • Seal Flask Boil
    • Remained Sterile

    "Heating the air destroyed life-giving properties of air"…
  7. How did Theodore Schwann contribute to Spontaneous Generation? (1800)?
    • Air could enter flask after passing through red-hot tube.
    • Sterile!!!
  8. What did Georg Fredric Schroder and Theodor Von Dusch contribute to Spontaneous Generation?
    • Sterile cotton wool in neck of boiled flask
    • Sterile!!!
  9. What did Felix Pouchet contribited to Spontaneous Generation? (1859)
    No air contamination, but still microbial growth!!!!!
  10. What did Luis Pasteur contribute to Spontaneous Generation? (mid 1800s)
    • Trapped stuff from air in sterile cotton
    • Put in sterile medium and stuff grew
    • swan neck flask!!! (scientific tool)
  11. How did John Tyndall contribute to Spontaneous Generation? (1877)
    • Dust carries germs
    • Get rid of the dust, broth sterile even if exposed to air.
  12. Main contributor to the Golden Age of Microbiology?
    *Germ Theory
    *Disease-causing organisms
    *Microbial control
    • Louis Pasteur (France)
    • Robert Koch (Germany)
  13. What are the contributions of Louis Pasteur to the Golden Age of Microbiology? (1822-1895)
    • Pasteurization
    • Vaccines
    • Chicken Cholera
    • Rabies
    • Anthrax
    • Small Pox
  14. How did Robert Koch contribute to the Golden Age of Microbiology? (1843-1910)
    • Isolated Anthrax agent: Bacillus anthraces
    • Koch's Postulates

    • 1.Microorganism must be present in every
    • case of the disease but absent from healthy organisms

    • 2.Microorganism must be isolated  and grown in pure
    • culture

    • 3.Same disease must result from inoculation of isolated microorganisms into healthy
    • organism

    • 4.Same microorganism must be isolated from
    • diseased host

    • •Also isolated causative
    • agent of Tuberculosis

    •Mycobacterium tuberculosis

    • •Nobel
    • Prize (1905)
  15. What's Molecular Koch's Postulates? (1988)
    • 1.“The phenotype or property. . . should be associated with pathogenic members of a
    • genus or pathogenic strains of a species”

    • 2.“Specific inactivation of the gene(s) associated with the suspected virulence trait
    • should lead to a measurable loss in pathogenicity or
    • virulence”

    3.“Reversion or allelic replacement of the mutated gene should lead to restoration of pathogenicity”

    4.Gene should be expressed during disease process

    5.Immune activity against gene product should protect