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What are the classification of Micro Organisms?
What are the 3 domains of life of the Woese Diagram?
What are Animalcules?
Older term for microscopic animals
What is Spontaneous Generation?
- Its the Phenomenon where 'living organisms [can] develop from nonliving matter'
- ex: meat can spontaneously produce maggots.
What did John Needham Discovered on Spontaneous Generation? (1748)
- Heated meat broth, then stoppered
- Became cloudy
- Orgamic matter contains vital force (turning nonliving into living)
What did Lazzaro Spallanzani Discovered on Spontaneous Generation?
- Seal Flask Boil
- Remained Sterile
"Heating the air destroyed life-giving properties of air"…
How did Theodore Schwann contribute to Spontaneous Generation? (1800)?
- Air could enter flask after passing through red-hot tube.
What did Georg Fredric Schroder and Theodor Von Dusch contribute to Spontaneous Generation?
- Sterile cotton wool in neck of boiled flask
What did Felix Pouchet contribited to Spontaneous Generation? (1859)
No air contamination, but still microbial growth!!!!!
What did Luis Pasteur contribute to Spontaneous Generation? (mid 1800s)
- Trapped stuff from air in sterile cotton
- Put in sterile medium and stuff grew
- swan neck flask!!! (scientific tool)
How did John Tyndall contribute to Spontaneous Generation? (1877)
- Dust carries germs
- Get rid of the dust, broth sterile even if exposed to air.
Main contributor to the Golden Age of Microbiology?
- Louis Pasteur (France)
- Robert Koch (Germany)
What are the contributions of Louis Pasteur to the Golden Age of Microbiology? (1822-1895)
- Chicken Cholera
- Small Pox
How did Robert Koch contribute to the Golden Age of Microbiology? (1843-1910)
- Isolated Anthrax agent: Bacillus anthraces
- Koch's Postulates
- 1.Microorganism must be present in every
- case of the disease but absent from healthy organisms
- 2.Microorganism must be isolated and grown in pure
- 3.Same disease must result from inoculation of isolated microorganisms into healthy
- 4.Same microorganism must be isolated from
- diseased host
- •Also isolated causative
- agent of Tuberculosis
What's Molecular Koch's Postulates? (1988)
- 1.“The phenotype or property. . . should be associated with pathogenic members of a
- genus or pathogenic strains of a species”
- 2.“Specific inactivation of the gene(s) associated with the suspected virulence trait
- should lead to a measurable loss in pathogenicity or
3.“Reversion or allelic replacement of the mutated gene should lead to restoration of pathogenicity”
4.Gene should be expressed during disease process
5.Immune activity against gene product should protect
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