Micro Lab Exam I

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Micro Lab Exam I
2014-02-05 17:47:03

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  1. Universal Precautions
    • handling of all items and specimens utilized in the laboratory as contaminated and infectious
    • (CDC) Centers for Disease Control
  2. Person Protective Equipment (PPE)
    • 1. Protective laboratory clothing
    • 2. Disposable gloves
    • 3. Eye protection
    • 4. Face masks
  3. Accidents, illness, and injures must be...
    reported immediately and recorded on the appropriate forms
  4. Possible routes of infection
    • 1. Airborne
    • 2. Ingestion
    • 3. Direct Inoculation
    • 4. Mucous membrane contact
  5. Airborne infection route
    Airborne: aerosols may form during shaking or agitation of bacterial cultures in liquid media. They also form when dropping and breaking a bacterial culture in liquid media.
  6. Ingestion infection route
    Ingestion: infection may result from failure to wash hands, eating, drinking, or chewing gum in the laboratory
  7. Direct Inoculation: infection route
    Direct Inoculation: infection may result from needle sticks or broken glass, in the laboratory
  8. Mucous membrane contact: infection route
    Mucous membrane contact: infection may occur if the organism can directly enter through mucous membranes such as through the conjunctiva of the eye
  9. Biohazard containers
    all potentially contaminated samples, specimen containers, pipettes, pipette tips, reaction vessels, stoppers, and such
  10. disposing of tooth picks, cotton swabs, and inoculating needles
    in a beaker with bleach
  11. slides which have come into contact w/cultures
    in a square container
  12. Types of Compound Light Microscopy
    • 1. Bright Field microscopy
    • 2. Dark Field microscopy
    • 3. Fluorescent microscopy
    • 4. Phase Contrast
  13. Bright Field Microscopy
    • Specimens that have pigments contrast with objects in the field of view can be seen
    • multipurpose for live and preserved stained specimens
    • note how specimens with few or no pigments have a low contrast cannot be seen with this microscope
  14. 2. Dark Field Microscopy
    • Uses a dark field condensor
    • with a "stop disc" (opaque disc) that keeps light from entering the objective
    • except peripheral light that is reflected off the sides of the specimen itself
    • can outline the organism's shape and is use for detecting Treponema pallidum (causes syphilus)
    • image result: brightly illuminated specimen that is surrounded by a dark field
  15. 3. Phase Contrast
    • bends light that passes through the specimen so that it contrasts with the surrounding medium (moving the light out of phase)
    • to alter the light that passes through diff parts of the organism
    • devices that transform the subtle changes in light waves passing though the specimen into differences in light intensity
    • allows internal structure to be seen
  16. 4. Fluorescent Microscopy
    • equipped with UV light that is directed towards the specimen, which has been stained with fluorescent dyes
    • to detect: Mycobacterium tuberculosis
  17. Immunofluorescence
    • flurorescent dyes are bound to specific antibodies
    • to detect:
    • 1. syphilus
    • 2. chlamydiosis
    • 3. trichomoniasis
    • 4. herpes
    • 5. influenzae
  18. Electron Microscopy types
    • 1. Transmission Electron microscopy (black n white)
    • 2. Scanning Electron microscopy