Micro Lab Exam I

• handling of all items and specimens utilized in the laboratory as contaminated and infectious
• (CDC) Centers for Disease Control

• 1. Protective laboratory clothing
• 2. Disposable gloves
• 3. Eye protection
• 4. Face masks

reported immediately and recorded on the appropriate forms

• 1. Airborne
• 2. Ingestion
• 3. Direct Inoculation
• 4. Mucous membrane contact

Airborne: aerosols may form during shaking or agitation of bacterial cultures in liquid media. They also form when dropping and breaking a bacterial culture in liquid media.

Ingestion: infection may result from failure to wash hands, eating, drinking, or chewing gum in the laboratory

Direct Inoculation: infection may result from needle sticks or broken glass, in the laboratory

Mucous membrane contact: infection may occur if the organism can directly enter through mucous membranes such as through the conjunctiva of the eye

all potentially contaminated samples, specimen containers, pipettes, pipette tips, reaction vessels, stoppers, and such

in a beaker with bleach

in a square container

• 1. Bright Field microscopy
• 2. Dark Field microscopy
• 3. Fluorescent microscopy
• 4. Phase Contrast

• Specimens that have pigments contrast with objects in the field of view can be seen
• multipurpose for live and preserved stained specimens
• note how specimens with few or no pigments have a low contrast cannot be seen with this microscope

• Uses a dark field condensor
• with a "stop disc" (opaque disc) that keeps light from entering the objective
• except peripheral light that is reflected off the sides of the specimen itself
• can outline the organism's shape and is use for detecting Treponema pallidum (causes syphilus)
• 
• image result: brightly illuminated specimen that is surrounded by a dark field

• bends light that passes through the specimen so that it contrasts with the surrounding medium (moving the light out of phase)
• to alter the light that passes through diff parts of the organism
• devices that transform the subtle changes in light waves passing though the specimen into differences in light intensity
• allows internal structure to be seen
• 

• equipped with UV light that is directed towards the specimen, which has been stained with fluorescent dyes
• to detect: Mycobacterium tuberculosis

• flurorescent dyes are bound to specific antibodies
• to detect:
• 1. syphilus
• 2. chlamydiosis
• 3. trichomoniasis
• 4. herpes
• 5. influenzae

• 1. Transmission Electron microscopy (black n white)
• 2. Scanning Electron microscopy