Virology 575: Lecture 5-DNA Viruses II

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Virology 575: Lecture 5-DNA Viruses II
2014-02-05 22:14:03
Virology 575

Virology 575
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  1. Splicing is common in _____ DNA viruses, but not in _____ DNA viruses
    Small, large
  2. Why is Adenovirus genome unusual
    It is linear DS genome
  3. Large DNA viruses, families
    herpesviridae, poxviridae, baculoviridae
  4. Large DNA viruses, general facts:
    DNA viruses with large dsDNA genomes, isometric or irregular capsids
  5. Large DNA replication properties
    • Use host transcription machinery early, viral later
    • Encode proteins required for DNA synthesis
    • RNA splicing is rare
    • Cause a variety of diseases
  6. Large DNA virus that carries its own replication machinery
  7. Large DNA virus that is not virulent to humans
  8. Expression of Herpes viruses
    • Temporal Cascade
    • Immediate Early genes: ICP0, ICP4, ICP27: major viral regulatory genes activated by VP16
    • Early genes: Enzymes involved in DNA synthesis, expression activated IE genes
    • Late genes: Structural genes, Capsid, Tegument, Envelope glyco, Expression activated IE/E genes, only expressed after DNA replication
  9. Themes of viral DNA replication
    • Faithful replication of a virus DNA genome requires unique mechanics, particularly at the ends of the genome
    • Synthesis begins at an origin
  10. Viral replication methods (directions)
    • Unidirectional (strand displacement): Adenoviruses, Parvoviruses, Poxviruses
    • Bidirectional (replication fork): Papillomaviruses, Polyomaviruses, Herpesviruses, Retroviral proviruses
  11. Where does viral DNA synthesis occur?
    • In replication centers
    •        -Formed in nucleus of infected cells