genetics chap 11 DNA replication
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what did the meselson-stahl experiment show?
that DNA was semi-conservative
what is a replicon?
length of DNA replicated following one initiation event at a single replication origin
what does DNA replication require?
template, all four dNTPs, Mg+2, ATP, DNA Polymerase
what is the function of gamma of DNA pol III, and the core enzyme? t shaped thing?
loads holoenzyme on template, elongates polynucleotide chain and proofreads, dimerizes core complex
what relieves downstream DNA supercoiling?
what is the role of primase?
synthesizes short fragments of RNA, which initiates transcription without free 3'
what degrades RNA primers?
5' to 3' exonuclease (DNA Pol I)
what is the role of DNA ligase?
joins lagging strand after primers are degraded
what is different about DNA polymerases?
differnt subunits of DNA POL has different functions, switches
how does recombination result
single strand breaks and rejoining heterologous DNA strands
what is the function of RecA
strand displacement in recombination to form a heteroduplex
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