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What did the fox say?
Ding ding ding
Which force are involved in maintaining the primary structure of a
one is NOT an example of supersecondary structure?
A. The pyrolle key
amount of energy released during a reaction tells nothing about the rate at
which that reaction will occur.
following methods are useful for cell homogenization:
of the above
E. All of the above
separates based on molecular weight?
or anion exchange
A. Gel Filtration
separation of a mixture of cations requires:
electrically neutral, but slightly polar substance
electrically neutral, nonpolar substance
B. An anionic substance
following are all principles of electrophoresis, except:
D. Binding to substrate
catalysts work by lowering the activation energy of the reaction.
the substrate concentration is low, the an enzyme reaction
display zero order kinetics
display first order kinetics
display second order kinetics
denature and cease to function
Will Display first order kinetics
lineweaver-burk plot is useful in the analysis of enzymatic reactions because
is easier to see if points deviate from a straight line, instead of a curve
allows you to calculate the exact values from a straight line
is an easy way to manipulate the data into a useful form
of the above
D. All of the above
1) The value of Vmax change in
of the above
B. Non-competitive Inhibition
1) Generally speaking, a competitive inhibitor
and the substrate cannot both bind to the enzyme at the same time.
1) The Michaelis-menton constant determines the
Vmax of an enzymatic reaction.
1) What types of macromolecules are usually
separated on agarose gel electrophoresis gels?
Nucleic Acids (DNA)
all enzymes proteins?
No but most are. There are some catalytic RNA's known.
are the elements of secondary structure in proteins? Pick one of these elements and outline in
detail the main characteristics of this form of secondary structure. Pay special attention to the forces involved,
and the arrangement of the structure.
- Alpha Helix:
- Stabilized by hydrogen bonds parallel to helix axis within backbone.
- R Groups point out from Helix
- B Sheet:
- Hydrogen Bonds are perpendicular to axis of sheet
- R Groups alternate above and below plane of sheet.
1) Explain in detail the significance of Km.
- Km is a measure of affinity for substrate and enzyme.
- Increase of Km = more dissociation and less affinity
- Decrease of Km = less dissociation and more affinity
a diagram of a basic tertiary structure, show three of the five forces that
stabilize this level of protein structure.
You MUST be specific, showing interacting atoms.
Look it up
the structural features of Secondary Structure in Proteins.
does the lock and key model of enzyme-substrate binding differ from the induced
- Lock and key model: substrate binds to
- that portion of the enzyme with a complementary shape.
- Induce fit model: Binding of the
- substrate induces a change in the conformation of the enzyme that results in a
- complementary fit.
in detail, how column chromatography work.
What components need to be present to run this sort of a
separation? Define all terms.
- Column chromatography works to separate out
- components of a mixture, through different interactions with a stationary
- phase. The stationary phase is typically a polymer with linkers attached to it.
- The stationary phase interacts with the components of the sampe and leads to separation.
- The mobile phase contains the mixture, usually Ph a buffer and passes over the
- stationary phase.