Bio 214 lab
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________ are th3e major macromolecular constituent of cells and are composed of one or more linear chains of ___________ linked together by peptide bonds in specific sequence
All twenty amino acids share a common structure called the
What technique did we use to allow cellular structure to be seen with the light microscope?
The diameter of the circle of light that you see when looking into a microscope is called?
Field of view
What stains the nucleus of a cell blue?
Name of the aquatic plant we used to observe cytoplasmic streaming
magnification and um's per ocular unit of 4x
magnification and um's per ocular unit of 10x
magnification and um's per ocular unit of 40x
another word for cytoplasmic streaming
Volumes for the micropipettes
what is the range of volume for a micropipette size p20?
what is the range of volume for a micropipette size p200?
what is the range of volume for a micropipette size p1000?
part of micropipette that allows you to adjust the volume that is measured
volume adjustment dial
part of micropipette that shows the volume that will be measure
What is the ul's of p20, p200 and p100 if the digital readout is: 070
- p20: 7.0ul
- p200: 70ul
- p1000: 700ul
What is the second stopping point on the plunger for?
Used for the complete discharging of solutions from the plastic tips
How much is each micropipette?
this measures how much light a sample absorbs
What is the special sample holder called that's used for the spectrophotometers?
- carboxyl group
- amino group
- hydrogen atom
what is the R group attached too?
analytical tool for distinguishing different biomolecules based on their chemical properties
what is the filter paper composed of for chromatography
how is the solvent drawn up on chromatography paper.
capillary action with hydrophobic organic solvent
what is the rate related too for molecules moving up the chromatography paper?
- affinity for the hydrophobic paper
- if the molecule goes further its more hydrophilic
What is the rate of movement of a biomolecule during paper chromatograph reported as?
- Relative mobility(Rf)
- distance/distance solvent moved
rule of thumb for handling chromatography paper
no bare hands
Is a benzene ring hydrophobic or hydrophilic?
How are polypeptides separated during SDS polyacrylamide Gell electrophoresis?
examples of housekeeping proteins
- enzymes of glycolysis
- proteins that package DNA
- cytoskeletal proteins
technique of separating charged particles in an electrical field
* passing an electrical current through some medium
which charged molecules move towards which charged electrodes on a SDS page?
- positively charged particles to the negative cathode
- negative to the anode
what are the three factors that control the relative rate of movement of a particle in general electrophoresis?
SDS-page stands for?
SDS polyacrylamide Gel Electrophoresis
what molecules, in respect to mass, shape and charge will move faster for general electorphoresis?
- less surface area
- greater total charge
How is SDS-page different from general electrophoresis?
it separates polypeptides based only on molecular weight
what is the gel-like medium through which the electric field is passed
what does SDS stand for?
sodium dodecyl sulfate
2 roles SDS plays in regards to the SDS page
- denatures the protein
- SDS molecules coat the protein giving it a uniform negative charge
the distance migrated by a polypeptide is inversely proportional to
the log of the polypeptides MW
How is the standard curve generated for the SDS-page
by using the stand proteins/housekeeping
the 5 tissues we took from the mouse for the SDS page
what does the instructor use to stain the proteins for the SDS page lab?
coomassie blue, for 1 hour
what is lactose formed from?
galactose and glucose
what must be hydrolyzed for lactose to yield its component monosaccharides so they can be absorbed?
what is the enzyme used for lactase and is the chemical analog of lactose
The lactose product that is absorbing the light
THe buffer for the lactose lab:
Stopper for the lactose lab
What does the DTT reducing agent do to proteins for the SDS page experiment?
break disulfate bridge which is covalently bonded, then they can run as separate proteins
How does glycerol help for the SDS page experiement?
- reduces diffusion during loading
- -its heavy & viscous
- - goes through gel straight
how does bromophenol blue help for the SDS page experiment?
- indicates when to stop
- shows where smallest molecules migrate
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