HematologyLab1Sickledex

Card Set Information

Author:
victimsofadown
ID:
265424
Filename:
HematologyLab1Sickledex
Updated:
2014-03-07 07:01:56
Tags:
Hematology
Folders:
Hematology
Description:
HematologyLab1Sickledex
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user victimsofadown on FreezingBlue Flashcards. What would you like to do?


  1. What is the genetic explanation behind Hgb S?
    Glutamic Acid is replaced by Valine in position 6 of the beta chain
  2. What does the Sickledex procedure test for?
    • The presence of Hgb S
    • Does not indicate amount, or homo/heterozygocity
  3. What is the principle behind the Sickledex test?
    • Sodium dithionite or sodium hydrosulfite reduces oxygen tension in the soln
    • Tactoids are formed when the O2 environment around the cell is reduced
    • This causes the characteristic "sickling" of the cells
    • Hgb S Is insoluble after exposure to soln, and the blood will be opaque
  4. What is the procedure (in detail) for the Sickledex test?
    • Mix the powdered contents and liquid reagents together
    • Place 3mL test soln into three test tubes in holder
    • Fill the pipette w/ capillary action to the black line
    • Put blood into one test tube, then invert the tube to thoroughly mix
    • Put the + control into one tube, and mix
    • Put the - control into the final tube, and mix
    • Allow to stand
    • View through solns for result.  If blood is opaque then the patient is Hgb S positive

What would you like to do?

Home > Flashcards > Print Preview