HematologyLab1Sickledex

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  1. What is the genetic explanation behind Hgb S?
    Glutamic Acid is replaced by Valine in position 6 of the beta chain
  2. What does the Sickledex procedure test for?
    • The presence of Hgb S
    • Does not indicate amount, or homo/heterozygocity
  3. What is the principle behind the Sickledex test?
    • Sodium dithionite or sodium hydrosulfite reduces oxygen tension in the soln
    • Tactoids are formed when the O2 environment around the cell is reduced
    • This causes the characteristic "sickling" of the cells
    • Hgb S Is insoluble after exposure to soln, and the blood will be opaque
  4. What is the procedure (in detail) for the Sickledex test?
    • Mix the powdered contents and liquid reagents together
    • Place 3mL test soln into three test tubes in holder
    • Fill the pipette w/ capillary action to the black line
    • Put blood into one test tube, then invert the tube to thoroughly mix
    • Put the + control into one tube, and mix
    • Put the - control into the final tube, and mix
    • Allow to stand
    • View through solns for result.  If blood is opaque then the patient is Hgb S positive

Card Set Information

Author:
victimsofadown
ID:
265424
Filename:
HematologyLab1Sickledex
Updated:
2014-03-07 12:01:56
Tags:
Hematology
Folders:
Hematology
Description:
HematologyLab1Sickledex
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