DNA sequencing: sanger method
- Prepare four tubes with the same DNA as the template, all four dNTP: A, T, G, C, DNA polymerase, and a DNA primer.
- Add concentration of a different ddNTP to each tube.
- Four sets of complememntary sequences are generated from template DNA.
- Separate the labled fragments by electrophoresis.
- Go down lanes and list the bases in the order they are fragmented.
- Use: for sequencing.