Lipid extraction in organic solvents:
- Neutral lipids: ethyl ether, chloroform, or benzene.
- Membrane lipids: polar organic solvents (ethanol or methanol), solvents reduce weak interactions.
- Method: phase extraction using chloroform:methanol:water. this is one phase and extracts all lipids. Add more water and it separates into two phases: water/methanol phase on top with proteins and sugars, organic phase on the bottom with lipids.