Classification of Microorganisms
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Subdivision used to classify organisms, e.g. domain, kingdom, phylum
The science of classification of organisms
The evolutionary history of a group of organisms; phylogenetic relationships are evolutionary relationships
Archaea include three major groups
- Methanogens-strict anaerobes that produce methane (CH4) from carbon dioxide and hydrogen
- Extreme halophiles-which require high concentrations of salt for survival.
- Hyperthermophiles-which normally grow in extremely hot environments
What is included in the kingdom of the Eukarya?
What is the domain that includes prokaryotes that do not have a peptidoglycan in their cell walls?
The Domain Archaea
The first name of the scientific name (binomial); the taxon between family and species.
The second or species name in a scientific binomial.
What is the binomial nomenclature?
The system that gives two names to each organism
A group of closely related organisms that breed among themselves.
The toxonomic hierachy
- All organisms
- Species - most specific
Prokaryotes are divided into two domains
Bacteria and Archaea
What does ELISA stand for?
Enzyme-linked immunosorbent assay
Why is an ELISA widely used?
It is fast and can be read by a computer scanner. Used in AIDS testing to detect the presence of antibodies against HIV.
What is Western blotting?
Used to identify antibodies in a patient's serum. Test for proteins and confirms HIV infection and Lyme disease
What is phage typing?
A test for determining which phages a bacterium is susceptible.
What is Southern Blotting?
A technique that uses DNA probes to detect the presence of specific DNA in restriction fragments separated by electrophoreis.
What does FISH stand for and what does it do?
fluorescent in situ hybridization; Fluorescent dye-labeled RNA or DNA probes are used to specifically stain microorganisms in place.
What are two types of serological test?
ELISA and Western Blot
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