Casein Hydrolysis/Phenylalanine Deaminase Test
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What type of agar is used in Casein Hydrolysis test?
- Milk Agar Plate
- -Casein (protein found in milk)
What enzyme breaks downs casein?
An exoenzyme named Casease
Describe what is happening in this test.
E Coli is (-) for the enzyme casease, therefore is unable to breakdown the casein in milk ager into smaller peptides & amino acids
B. cereus is (+) for the enzyme casease, therefore is was able to break down the casein into small peptides & amino acids for cell use
What does a Phenylalanine Deaminase Test determine?
Tell you if a cell is capable of producing a an enzyme called deaminase.
If an organism is (+) for enzyme deaminase what happens?
It strips the amine group (NH2) from the phenylalanine agar creating phenylpruvic acid.
How to tell is an organism is (+) for deaminase
You add 4 drops of ferric chloride (FeCl3
- (+) for deaminase turns green
- (-) for deaminase stays the same color
The removal of an amine group (NH2) from phenylalanine(AA)
If an organism is (-) for deaminase, and you add 4 drops of ferric chloride
It turns yellow which mean no phenylpyruvic acid/ No phenylalanine deaminase enzyme
What test is this, what is it looking for, what does it mean?
- Phenylalanine Deaminase Test
- Green = Positive for Phenylalanine Deaminase
- Yellow/Same color = Neg for Phenylalanine Deaminase
Ferric Chloride is used to detect any Phenylpyruvic acid that is made. If organism has the enzyme to break down phenylalanine = positive
What is Phenylalanine?
Its an Amino Acid
When deaminated the amine group NH2 is removed
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