Bio 214 Lab

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Author:
lacythecoolest
ID:
268705
Filename:
Bio 214 Lab
Updated:
2014-03-31 23:41:22
Tags:
lab
Folders:
BIO 214
Description:
Lab practical II
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  1. the major energy producing process in eukaryotic cells
    aerobic respiration
  2. structure of mitochondria
    • outer membrane
    • folded inner membrane
    • fluid-filled compartments
  3. houses the many enzymes essential to the chemical rxns of respiration
    mitochrondria
  4. with FAD what rxn does succinate dehydrogenase catalyze?
    Succinate to Fumerate
  5. flavin adenine dinucleotide
    FAD
  6. where are succinate dehydrogenase and coenzyme FAD located?
    tightly bound to inner membrane of mitochondria
  7. What does FAD do to succinate in the electron transport chain
    • removes 2 hydrogens/electrons
    • transfers to coenzyme Q
    • go to ATP
  8. what is used to visualize and measure the rate of succinate dehydrogenase/FAD rxn?
    DCIP
  9. artificial electron acceptor
    DCIP
  10. what happens when DCIP changes from blue to colorless?
    it accepts electrons from E-FADH2
  11. what is the product of succinate dehydrogenase/FAD rxn?
    E-FADH2
  12. What needs to happen for the DCIP to successfully compete for the electrons from E-FADH2?
    • normal pathway of electrons is blocked by sodium azide in electron transport chain
    • blocks the final transfer of cytochrome a3 -> O2
  13. poison used to block electrons
    sodium azide
  14. what are we measuring in the succinate dehydrogenase/FAD rxn
    the decrease of oxidized DCIP(blue) to colorless absorbance at 600nm over 35 minutes
  15. The kinetics related to succinate dehydrogenase/FAD rxn
    Michaelis-Menton kinetics
  16. explain Michaelis-Menton Kinetics
    Velocity of rxn increases proportionally to an increase in enzyme concentration

    all factors constant
  17. what suspension will we be using for enzyme(succinate dehydrogenase) concentrations
    mitochondria suspension
  18. slope of the Michaelis-Menton kinetics graph
    Absorbance v Time graph
  19. additional enzyme concentration/competitive inhibitor test for succinate
    malonate
  20. a structure that is similar to the substrate
    competes for binding to active site on enzyme
    competitive inhibitor
  21. a liquid; floating on the surface above a sediment or precipitate
    suernatant
  22. where is the mitochondria found after the 1st centrifuge
    supernatant
  23. where is mitochondria found after the second centrifuge
    pellet

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