micro bio lab prac 2

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micro bio lab prac 2
2014-04-03 18:14:17

antimicrobial drugs, carbohydrate catabolism, fermentation, protein catabolism, respiration
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  1. antibiotics
    microorganism that inhibits other microorganisms
  2. antimicrobial drugs
    chemical used internally
  3. MIC
    (minimum inhibitory concentration) to test bacteria growth in dilution of antibiotic in nutrient growth
  4. bacteriostatic
    bacteria growth
  5. bacteriocidal
    no growth
  6. carbohydrates
    chemical reaction that releases energy from decomposition
  7. catabolism
    molecule that contains carbon, hydrogen, and oxygen
  8. hydrolytic enzymes
    leaves the cell and breaks down, by adding h2o
  9. differentiate oxidative catabolism and fermentative catabolism.
    • oxidative catabolism=oxygen is presence. 
    • fermentative catabolism= doesnt require oxygen, but may occur in its presence
  10. fermentation
    an anaerobic (w/o oxygen) cellular process in which organic foods are converted into simpler compounds, and chemical energy is made.
  11. Starch Hydrolysis will be _________. Unchanged will be _________ after adding gram's iodine.
    • clear
    • dark blue
  12. OF-Glucose:

    ________ color = uses glucose aerobically.
    ________ color = ferments glucose
    ________ color = doesnt use glucose
    • yellow top
    • yellow
    • blue/green
  13. carbohydrate fermentation test: (w/ phenol red)

    _________= neutral/alkaline solution
    _________= acid is presence
    _________= gas is presence
    • red
    • yellow
    • cloudy yellow
  14. protein hydrolysis: (gelatin hydrolysis)

    to liquify
    warm water at 50 degree C
  15. protein hydrolysis: (gelatin hydrolysis)

    to solidify
    heat at 25 degree C or place tube in crushed ice.
  16. protein hydrolysis: (urease test)

    positive urease wil turn ___________.
  17. protein hydrolysis: (urease test)

    negative urease will turn ___________.
    • yellow
    • or at pH 8.4 or higher = fuchsia
  18. protein hydrolysis: 

    removal of amino group
  19. protein hydrolysis:

    removal of carbon dioxide from amino acid
  20. protein hydrolysis: (phenylaianine deamination)

    after adding 4-5 drops of ferric chloride reagent, if _______ colored, it is positive.
    dark green
  21. protein hydrolysis: (MIO)

    ornithine decarboxylation reaction =

    negative =

    after Kovacs reagent, a positive indole =
    • purple
    • yellow
    • cherry red
  22. respiration reduction
    electron passed from one molecule to another (one gaining e- = reduce; losing e- = oxidised)
  23. aerobic respiration
    requires oxygen in order to generate energy
  24. anaerobic respiration
    oxygen is absent/scarce
  25. respiration: (nitrate reduction)

    if gas is obscured, it is __________.
  26. in Catalase test, bubble indicate _______.
    positive catalase
  27. VP Test:

    pinkish red indicates _______.
    positve V-P test
  28. citrate test:

    green indicates ____________.
    citrate negative