Thioglycollate Broth/ pH/ Osmotic pressure/ temp
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What are two agents in Thioglycollate Broth?
- Sodium thioglycollate
A reducing agent that removes available oxygen.
Found in Thioglycollate broth
- An oxygen indicator
- Pink= Oxygen presents (Oxidized)
- Clear = Oxygen absent (Reduced)
What is the main use of thioglycollate broth?
Its used to determine the oxygen requirements in organisms
Acidophile and their pH range
Grows best at pH lower then 5.5
Neutrophile and their pH range
- Grows best in neutral environments
- pH 5.5 - 8.5
Alkaliphile and their pH range
Grow best at pH 8.5 and above.
The movement of molecules down its concentration gradient
The movement of water molecules across a semipermeable membrane, down its concentration gradient
Effects of Hypotonic solutions
- Means less [solute] outside the cell
- Has a net movement of water into cell
Effects of Isotonic solution
- When the [solute] is equal inside the cells and outer environment
- Equal movement in and out of the cell
Effects of hypertonic solution
- When [solute] is higer outside the cell
- Causes water to move out of the cell
Bacteria that can frow in [salt] of 3% or more
Below 20o C
Organisms who grow best between 15oC and 45oC
Organisms that grow at 40oC and higher
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