Chemical Digestion

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Author:
Shira
ID:
270715
Filename:
Chemical Digestion
Updated:
2014-04-15 20:46:01
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Bio Lab
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Questions pertaining to the Chemical Digestion Lab Powerpoint
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  1. __?__ is the process of breaking down food with the addition of water.
    Hydrolysis
  2. The chemical the digestive enzyme is breaking down is the __?__.
    substrate
  3. What the substrate is broken into is the __?__.
    product
  4. Why are controls done?
    To make sure reageants are functioning properly and are not contaminated.
  5. If amylase breaks down starch what is the substrate? The product?
    Starch; Maltose (disaccharide)
  6. When starch is present, Iodine Potassium Iodine (IKI) stains starch what color?
    Black
  7. When starch has been broken down with the addition of Iodine Potassium Iodine (IKI), the contents in the tube will appear what color?
    Dilute iodine (orange color)
  8. What is Benedict's reagent testing for?
    A type of sugar such as Maltose.
  9. When Benedict's reagent is added to a tube after boiling, the color(s) will be __?__ if sugar (Maltose) is present.
    green, red, or orange
  10. When Benedict's reagent is added to a tube after boiling, the color(s) will be __?__ if sugar (Maltose) is not present.
    blue (no change; color of Benedict's reagent)
  11. Will an enzyme work at a pH other than its optimum pH?
    Sometimes, depending on the pH and the enzyme. However, enzymes work best at their pH optima.
  12. What will boiling do to an enzyme?
    Boiling denatures enzymes so it means it changes the structure of the enzyme causing it not to work anymore.
  13. What will freezing do to an enzyme?
    It only slows down the activity (it does not stop it from working).
  14. What enzyme digests carbohydrates into disaccharides?
    Amylase
  15. What pH does Amylase works best in? Where in the body would this enzyme work best?
    7.0; in the mouth (also in the small intestine)
  16. What enzyme breaks down proteins?
    Pepsin
  17. What pH does Pepsin works best in? Where in the body would this enzyme work best?
    2.0; in the stomach (activated by HCl)
  18. What instrument is used to see if proteins (BAPNA in lab experiment) are broken down? What color should be seen when using this instrument?
    Spectrophotometer; a yellow color should be seen when this instrument shines a light through the solution.
  19. What enzyme breaks down fats into monoglycerides and fatty acids?
    Lipase
  20. What pH does Lipase works best in? Where in the body would this enzyme work best?
    7.0; secreted by pancreas and active in the small intestine.
  21. What is the function of bile? How does this function help with lipase efficiency?
    Bile emulsifies fats; this provides more surface area and increases lipase efficiency.
  22. What should happen when Lipase breaks down fat?
    Fatty acids are released and an increase in fatty acids in the solution will make the solution more acidic.
  23. What instrument is used to measure the pH of the solution?
    pH meter

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