WMU BIO LAB FINAL!

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berraL13
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270726
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WMU BIO LAB FINAL!
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2014-04-15 23:47:05
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wmu bio lab final
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wmu biol labe final
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  1. decaboxylation test attacks on the _____ from the _____. Lowers _____ of media
    • carboxy, amino acid. 
    • pH
  2. Phenylalanine Deamination test abibly to produce. Attacks _____ in the amino acid.
    deaminase. Amine
  3. phenylalanine deaminase test reacts with _____ with Phenylpyruvic acid and does what…
    ferric chloride and turns dark green
  4. what test is this

    • phenylalanine deamination
    • left negative right positive
    • decarboxylation test.
    • left positive right negative
  5. Urea hydrolysis looks for what enzyme 
    produces the _____ of amino acids
    lowers..
    • urease 
    • decaboxylation 
    • pH
  6. Gelatin hydrolosis for what enzyme
    gelatinase
  7. SIM
    • S: break down cysteine.
    • I: tryptophan break down into pyruvate by tryptophanase. kovacs reagent 
    • M; able to move throughout culture
  8. Effect of osmotic pressure. Not NaCl (3)
    • Hyposmotic: h2o moves in 
    • hyperosmotic:h2o moves out 
    • isosmotic: in and out
  9. effect of osmotic pressure. NaCl (3)
    • halophiles: 3% or higher
    • extreme halophiles: 15-25% 
    • osmotolerant: range
  10. effect of pH organisms (3)
    • acidophiles: ph < 5.5 
    • neutrophiles: 5.5-8.5
    • alkaliphiles: pH> 8.5
  11. effect of temp (5)
    • psychrophiles <20
    • psychrotrophs: 0-30
    • mesophiles 15-45
    • thermophiles >40
    • extreme thermophiles 65-110
  12. types of ultraviolet light
    which is most harmful and why
    • UV A,B,C. 
    • UVC, because is changes DNA and causes dimers
  13. how many colonies in a countable plate
    25-300 cfu.
  14. serial dilution is a _____ method
    indirect.
  15. three common dilution methods
    • streak plate
    • pour plate
    • spread plate
  16. PEA (phnylethyl Alcohol Agar)
    selctive for what and inhibit what
    • selective gor gram positive streptococcie, staphylococci, and entrococco. 
    • inhibit gram negative growth.
  17. left gram (+0 right gram (-)
  18. Mannitol Salt Agar (MSA)
    selective for what, how much salt
    • selectis for gram positive.
    • 7.5% salt
  19. MSA ( Mannitol Salt Agar) Growth
    Yellow grow W/ yellow halo
    pink growth
    no growth
    • - positive for mannitol fermentation (G+)
    • - negative for fermentation (g+)
    • - probably gram negative
  20. MSA

    • YY: fermentation
    • pink: no fermentation G+
    • no growth: gram negative
  21. Bile esculin contains what media. Selects for what
    • ferric citrate
    • Group D streptococcus and enterococcus
  22. bile esculin growth
    black
    non (brown)
    • hydrolize bile esculin
    • no hydrolyze
  23. Bile esculin

    • left positive to hydrolyze bile esculin 
    • right negative cannot hydrolyze
  24. EMB( Eosin Methlene Blue Agar) slecets for what
    • sugar(lactose/sucrose) allow growth for fecal coliform 
    • inhibit gram positive. DYES.

  25. EMB
    • Left coloform ferment lactose
    • right coliform ferment lactose 
    • bottom gram positive
  26. Hektoen Enteric Agar (HE) isolate what
    gram negative
  27. HE 
    • Top: lactose ferment
    • Right: non ferment, sulfur reduction
    • Botom: gram negative (cannot ferment or reduce)
    • Left: gram positive
  28. Macckonky agar contains what
    lactose, bile salts, indicator dyes

  29. MacConky Agar
    • 1: not inhibited by bile (g-) kind ferment lactose
    • 2. Not inhibited by bile, ferment lactose. (g-) 
    • 3. inhibited by bile (g+) 
    • 4. not inhibited by bile, no ferment lactose (g-)
  30. define disinfectant
    reduce number of microbes on non-living surfaces.
  31. define antiseptic
    germicide used on living tissue
  32. zone of inhibition
    bacteria sensitivity to antibiotic
  33. define bacterialcidal
    kill organism
  34. define bacteriostatic
    inhibit organism
  35. organism used to make sauerkraut
    lactobacillus spp
  36. organism used to make ginger ale
    bakers years
  37. organism used to make yogurt (2)
    lactobacillus bul. and streptococcus therm.
  38. organism that makes vinegar
    saccharomyces
  39. pathogen
    cause disease
  40. define coliform
    • falcutative areobe/ areobic
    • gram negative
    • lactose fermenter
  41. multiple tube test
    fecal coliforms
  42. serial dilution is for what test
    microbes in food
  43. propioniobacterium 
    and why so special
    • live in hair follicles. maintain pH of skin 3-5
    • usually bacteria on skin is g+ and salt tolerant
  44. what agar do we put skin sample on
    MSA (mannitol Salt Agar)
  45. What organism is on this MSA plate
    Staphylococcus Aureus
  46. normal flora of skin
    gram + salt tolerant and resist drying and secretions of oil glands
  47. upper respiratory flora v Lower
    Upper has micro flora. lower is supposed to be sterile. or anaerobe microbes
  48. antagonism in lower reps. tract
    suppressing growth of other organisms in competitions of nutrients and production os inhibitory substances.
  49. what agar is used for hemolysis
    Blood Agar
  50. the throat has which main species
    streptococcus
  51. what species produces hemolysin's
    streptococcus; which destroy red blood cells
  52. three types of hemolysin
    • alpha; green and middle 
    • beta; clear zone: bad 
    • gama; no color and healthy
  53. hemolysin and what agar
    • left: beta/ bad 
    • middle: alpha middle
    • right: gamma/ good
  54. bacteria in large intestine
    • enterobacteriace 
    • lactobacillus 
    • enterococcus 
    • bifi
    • bacteroides
  55. GI infections usually cause from...
    ingestion of food and water
  56. GI tract gets plated on what
    McConkey Agar with poop to find g- coliforms
  57. TSIA agar has what amount of glucose and lactose/sucros
    .01, 1%
  58. TSIA reuslts
    • a ferment glucose but not S/L 
    • b. produce ammonia 
    • d. h2S production 
    • e. acid produce
  59. positive result for UTI
    • >1000 same species
    • > 100 coliforms
  60. equation for Urine
    CFU/ loop volume 

    loop volume .01 unless given.
  61. the 4 classes of fungi
    • Zygomycetes
    • ascomycetes 
    • vasidiomycetes
    • deuteromycetes
  62. The 2 structures on fungus
    • hyphae: long part
    • spore: circle part
  63. structure on yeast
    pseudohyphae

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