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Process that destroys or removes all viable microorganisms (including viruses and endospores)
Physical process or chem agent to destroy vegetative pathogens but not bacteria
endospores (Bleach, Iodine, Boiling heat) (normally used on inanimate objects)
Cleansing techniques that mechanically removes microorganisms as well as other debris to reduce contamination; important in resturants, dairies, common usues for
cooking dishes, etc. (soaps, detergent, dish washers)
Process in which it reduces the number of microbes on human skin (alcohol, surgical hand scrubs)
Microbes on our skin. Examples:
- Normal biota
- Micrococcus lutes, staphylococcus epidermidis, Staph aureus, corynebacterium, and
- propionibacterium acnes.
What are transient organisms?
Organisms that are in competition with the normal biota but do not grow there, acquired by contact with contaminated surfaces andare usually easily removed, some cause disease.
3 major components of handwashing
First step: soap (process called saponification, which involves treating a fat with a base in the presence of heat)
- Second step: friction (helps physical
3rd step: incorporate an antimicrobial agent called an antiseptic in soap (helps kill some of the remaining organisms without harm the skin)
How does a surfactant (i.e. soap) work to ridus
Surfactants – disrupt the lipid portion of cell membranes
What are some of the limits of surfactants like
Limits – some bacteria have waxy cell walls that resist surfactants, endospores
What are some of the limitations of using UV light to kill microbes?
- Can’t penetrate plastic, can only be used on
- surfaces, air and water, not good on living tissue
anything that kills or inhibits the growth of microorganisms
a chemical made by microogranisms to kill or inhibit other microorganisms (penicillin)