Home > Flashcards > Print Preview

The flashcards below were created by user victimsofadown on FreezingBlue Flashcards. What would you like to do?

  1. What are the cell stages of Lymphocyte development and maturation?  Where do they occur?
    • Lymphoblast
    • Prolymphocyte
    • Lympocyte
    • Occurs in the lymph node
  2. What are the criteria for identifying Lymphocytes?
    • Morphological classification (small, medium/large)
    • Functional classification (T, B, NK)
    • Operational classification (response to mitogens, surface markers, rosette formation in sheep's blood)
  3. What are the surface markers found on various T and B lymphocytes?
    • T cells: CD3, CD4, CD8
    • B cells: CD19, CD20, CD22
  4. What are the basic functions of each type of lymphocyte? Name the subtypes w/ surface markers and give their basic function?
    • T cells: cell mediated response
    • Inflammatory- recruit macrophages and neutrophil
    • Cytotoxic (CD8)- kill pathogens (viruses)
    • Helper T cells (CD4)- TH1 participate in cell-mediated immunity
    • Helper T cells (CD4)- TH2 participate in antibody-mediated immunity by activated B cells
    • B cells: humoral response
    • plasma cells
    • memory cells
    • NK cells: single functioned, no subsets
  5. What are the elements of the lymphatic system?
    • Primary organs: hematopoetic blood islands
    • fetal liver
    • bone marrow
    • thymus
    • Secondary organs and tissues: Lymph nodes
    • spleen, tonsils
    • Peyer's patches
    • anal opening
  6. What is the function of the lymphatic system? Describe the "flow" of lymph throughout the body using specific names
    • drainage of interstitial fluid and transport of foreign substances
    • Vessels are blind-ended and fluid moves via peristalsis
    • thoracic duct: collects lymph from entire left side and bottom right side of body
    • delivers fluid to the left subclavian vein
    • right lymphatic duct: collects lymph from upper right side of body (head, chest, neck)
    • delivers lymph to the right subclavian vein
  7. What is the percentage breakdown of B and T cells in the peripheral blood?  The thoracic duct?
    • Peripheral blood: 60-80% T, 20-30% B
    • Thoracic duct: 85-90% T, 10-15%
  8. What specific location are B and T cells (different for each) found in the lymph nodes?  The spleen?
    • T cells in lymph nodes: paracortical regions, lymphoid follicles
    • B cells in lymph nodes: Germinal centers, subcapsular region, medullary cords
    • T cells in spleen: periarteriolar sheaths
    • B cells in spleen: germinal centers, periphery of periarteriolar sheath, red pulp
  9. Describe the difference in kinetic and circulatory patterns of B and T cells
    • B cells: mostly short lived
    • T cells: mostly long lived, rapid transmit time in blood, long G0 phase
  10. T cell education location?  Process of maturation?  Substances produced?
    • Educated in thymus (after creation in marrow)
    • Recognize antigen and dedifferentiate 
    • Become effector (helper or killer) cells as well as acting as inflammatory cells
    • Produce communicative substances (transfer factor, lymphotoxin, interferon, migration inhibiation factor, migration activation factor)
  11. B cell education location?  Process of maturation?  Substances produced?
    • Educated in bursa equivalent in marrow or peyer's patches
    • Dedifferenation stimulated by antigen OR T cells
    • Become plasma or memory cells
    • Produce humoral antibodies (A, D, E, G, M)
  12. What are the functions of each immunoglobulin?
    • A: cidal and found in secretions *dimer
    • D: function uncertain
    • E: binds to basophils and mast cells sensitizing them for certain allergic reactions
    • G: primary Ig, transferred across placenta
    • M: first antibodies to appear *pentamer
  13. Describe the evaluation of neutrophil function
    • Absolute neutrophil count: increased during infection
    • Neutrophil morphology: larger during infection
    • Evaluation of β-integrins (CD11, CD18): aids in diapedesis
    • Evaluation of neutrophil oxidative burst
  14. Describe evaluation of complement
    • Total hemolytic complement activity (CH50)
    • Specific component testing
    • Immunoassay for specific components
    • Functional testing for specific components
  15. Describe B, T, and NK cell screening and secondary tests
    • B cell screening: Quantitative immunoglobulins (via titers)
    • specific antibody
    • T cell screening: HIV testing
    • lymphocyte count
    • delayed-type hypersensitivity skin tests
    • B cell secondary: B cell enumeration (flow cytometry)
    • in vitro B cell function tests
    • T cell secondary: T cell enumeration (flow cytometry)
    • T cell proliferation (mitogen, antigen)
    • T cell cytokine production
    • T cell cytotoxicity
    • NK testing: NK cell enumeration (flow cytometry)
    • In vitro functional cytoxicity assay
  16. What are the three diseases of the Lymphocytes w/ VERY brief description of symptoms
    • DiGeorge Syndrome: little T cell function, hypocalcemia, and congenital heart disease
    • Burton's Agammaglobulinemia: failure to produce mature B cells and gamma globulin
    • Severe Combined Immunodeficiency Disease (SCID): Lack of function of both T and B cells
  17. DiGeorge Syndrome symptoms? cause? Notable information? Treatment? Outlook?
    • Little or no T cell function, hypocalcemia, and congenital heart disease
    • Immunodeficiency due to hypoplasia of the thyums
    • Hypocalcemia due to hypoplasia of the parathyroid glands
    • Genetic disorder (microdeletion in chromosome band 22q11.2)
    • Most common genetic cause of congenital heart disease next to Down syndrome
    • Treatment is thymus tissue transplant
    • Death by age 2 without treatment
  18. Burton's Agammaglobulinemia symptoms? cause? Notable information? Treatment? Outlook?
    • Failure to produce mature B cells and gamma globulin
    • Genetic disorder (X-linked recessive trait, mutation in tyrosine kinase gene Xq21.3-q22)
    • Prone to bacterial, but not viral, infections
    • Treatment is IV gamma globulin and antibiotics
    • Death within first 10 years without treatment
  19. Severe Combined Immunodeficiency Disease symptoms? cause? Notable information? Treatment? Outlook?
    • Little or no adaptive immune function (Both B and T cells affected)
    • Various genetic defects...
    • X-linked SCID
    • ADA deficiency
    • Purine nucleoside phosphorylase (PNP)
    • MHC class II or bare lymphocyte deficiency
    • "Boy in the bubble disease"
    • Very rare (1/500k births)
    • Death within first year of life if untreated

Card Set Information

2014-04-24 08:37:17
Show Answers:

What would you like to do?

Home > Flashcards > Print Preview