ATP synthesis- F-type ATPase. Peripheral membrane protein (first oxidative factor identified, 1 in F1 = first). F1 can be purified away from Fo, isolated F1 can catalyze ATP synthesis. F1 contains 5 different subunits. 3 alpha and 3 beta are alternating, the beta subunits have the catalytic site. realize that the F1 is inside the matrix of the mitochondria. Gamma subunits only bind one beta subunit at a time, it is the central "pole" looking component. The beta subunits are all in different conformations, each one binds different things on a cyclic basis: one subunit doesn't bind anything, another subunits binds ADP, and another subunit binds ATP.