Microbiology Test 1
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Microbiology Test 1
The optochin test distinguishes between which two bacterial groups?
and alpha-hemolytic Streptococci.
Out of the alpha-hemolytic Streptococci species, which one is normally inhibited by optochin?
SXT stands for the Sulfamethoxazole-Trimethoprim sensitivity test. What is the name of the antibiotic in this test?
In the Bacitran test (SXT), what are the two groups that are distinguished?
Between group A beta-hemolytic streptococci and non group A beta-hemolytic streptococci.
In the Bacitran test (SXT), which group of beta-hemolytic Streptococci are sensitive to bacitran and which group are resistant?
Group A are sensitive to bacitran while most non-group A are resistant.
For the PYR test, what color designates a positive?
The PYR test is used to identify a presumptive positive for what?
Group A Streptococci
When using PYR to identify a presumptive positive for Group A Streptococci, which other species will come up positive for this test?
What causes food poisoning in the body via
Mannitol Salt agar is selective for which organism?
Staphylococci and Streptococci are which type of organism? (Gram + or -)
Which test do you use if you want to distinguish between Staphylococci and Streptococci?
Out of Staphylococci and Streptococci, which organism is catalase negative?
Which Staphylococci ferments mannitol?
What has been added to mannitol salt agar that inhibits bacteria other than S. aureus?
Modified Thayer-Martin agar is selective for which bacterial species?
is gram positive or gram negative?
What is the component in Modified Thay-Martin agar that kills all gram negatives except
What is the component in Modified Thay-Martin agar that kills fungi?
What is the component in Modified Thay-Martin agar that inhibits gram negatives which include the prevention of swarming Proteus?
These organisms are formally called Nutritionally variant streptococci and require what for growth?
Pyridoxal or vitamin B
Nutritionally variant streptococci are and organism that only grow around the colonies of other microbes. What is this process called?
This form of Staphylococcus is pleomorphic and is
negative for coagulase
is often confused with
is PYR what?
What are the virulence factors of
1. Clumping factor (clots proteins that surround organism)
2. Leukocidines (kills WBCs)
3. Protein A (bins Fc instead of Fab, causes inhibition of opsinization and phagocytosis)
4. Superantigen (overactivates T cells)
What is the cause of necrosis in MRSA?
PVL (Panton-Valentine Leukocidin)
What does Beta-lactamase test for resistance to?
What is considered a positive and negative test result for Beta-lactamase?
: No color
MacConkey's agar is supportive for which organisms?
Gram negative bacilli
MacConkey's agar utilizes which type of fermentation?
For the CAMP test, how do you want to incubate the media?
In ambient air
MacConkey's agar is selective, differential, or both?
Both, it selects for gram negative bacilli while inhibiting the growth of gram positive with crystal violet and bile salts.
Which group of Strep organisms produce the camp factor?
This gram negative organism ferments glucose, but
maltose, lactose, sucrose, or DNAse?
During the Sodium Hippurate procedure, hippuric acid is hydrolyzed by hippuricase to produce which two byproducts?
Glycine and benzoic acid
During the Sodium Hippurate procedure, what reagent is used to detect the end product glycine to produce a blue color if positive?
During the Sodium Hippurate procedure, how long after incubation do you add the ninhydrin reagent?
Vancomycin inhibits which gram type of organisms?
Which are the
of the two organisms?
Moraxella or Neisseria?
What is the compound used in the oxidase test is oxidized by cytochrome c in the presence of oxygen to produce a purple color?
Which compound is used in the bile solubility test?
Which Streptococcus is soluble in bile salts while other alpha hemolytic strep are not?
has a requirement for what type of an environment?