microbiology lab possibles

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  1. Know where the emergency eye wash & shower are located & how to use them.
    lab safety
  2. , Ignaz Semmelweis (1815-1865), an obstetrician made some observations in the 1840’s of patients from the Vienna hospital clinic used by medical students and doctors versus a clinic used by midwives.1
  3. an obstetrician made some observations in the 1840’s of patients from the Vienna hospital clinic used by medical students and doctors versus a clinic used by midwives.1
    , Ignaz Semmelweis (1815-1865),
  4. how often should one wash hands
    Most people would agree that hands should be washed between patients, and between procedures and touching patients
  5. Someone does not have to be sick or asymptomatic  to be colonized with bacteria.
  6. VRE survived for up to 7 days on a countertop”3
  7. List some reasons why health care workers should always practice correct hand hygiene.
    So the spread of bacteria and viruses can be greatly reduced.    Also to prevent spread of disease to the healthcare worker
  8. How long can VRE potentially stay alive on an inanimate surface?
    It can stay alive for up to 7 days on a inanimate surface.
  9. How often should a person clean their hands after touching a patient?
    Immediatly after handeling bodily fluids and between each patient to prevent the spread of disease.
  10. How often should a person clean their hands after touching a contaminate surface?
    The hands should be washed immiedatly after touching a contaminate.
  11. Identify some reasons why people do not wash their hands.
    They get in a hurry between patients and forget to or when under stressful situations one may forget to wash after touching a contaminate
  12. Can a health care worker who touches someone who is colonized with microorganisms but is not infected cause other people to become sick? Explain your answer.
    Yes because some people may be immune to the microorganism or the symtoms may not show yet and they can easily be passed on to someone else and infect the other person.
  13. ⦁    What is the main purpose of the simple stain technique
    This is to get an idea of the simple shape and to identify the shape of the microbes.
  14. ⦁    Which dye(s) are used for the simple staining technique
    Basic dyes are methylene blue, basic fuchsin, and crystal violet
  15. ⦁    Why are basic dyes sometimes called cationic dyes and acid dyes sometimes called anionic dyes?
    Cationic dyes are positively charged and bind with the negative charges . Acid or anionic dyes have negative charges and will not easily stain microbes due to electrostatic repulsion
  16. . Why are basic dyes used to stain microbes rather than acidic dyes?
    A more acidic stain could damage the cell causing the image to be distorted or not stick onto the plate at all.
  17. List 3 examples of basic dyes used to stain microbes.
    1. crystal violet2. basic fuchsin3. methylene blue
  18. What does the simple stain allow you to observe?
    This allows you to see the shape of the microorganism and easily identify them or at least what group they belong to.
  19. You have isolated an unknown bacterium and want to make some morphological observations such as microscopic shape and arrangement. You find some hematoxylin-eosin stain on the bench and apply it to your bacteria. When trying to observe the microbe under the microscope you discover the cells of bacteria are clear. Using your knowledge of stains, explain this observation.
    There could have been an error in the process of taking the sample. Such as when the plate was passed through the flame, it was left on too long and it caused the microbe to burn off. Another factor could have been because the more acidic dye does not easily adhere to the cells due to electrostatic repulsion.
  20. . In lab you have been dying bacterial smears. You have a wool knit sweater at home that you would like to dye dark blue. You try using methylene blue since you have been working with that in lab only to find the dye does not seem to adhere to the sweater. Again using your knowledge of stains, explain this phenomenon.
    The methylene blue is a basic dye and wil not adhere to textiles. A more acidic dye -such as hematoxylin-eosin is more made for fabrics.
  21. ⦁    What color will S. aureus possibly be if the alcohol is not applied?
  22. ⦁    What color will E. coli possibly be if the alcohol is not applied?
  23. ⦁    For bacterial cells, discuss what determines the gram stain reaction.
    peptidoglycan in the cell walls and whether it is Gram pos or Gram Neg.
  24. ⦁    Can you detect contamination when you are looking at a gram stained sample?
    If there is a contamintation it could potentially result in a different color depending if it is Gram positive or Gram negative.
  25. From
    research published in "Clinical Infectious Diseases and Infection
    Control and Hospital Epidemiology", how long did vancomycin resistant
    enterococci survive?
    7 days
  26. Hands, gloves and bodies could be colonized with vancomycin resistant enterococci.
  27. Who suggested doctors and students should wash their hands with chlorinated lime solution?
  28. Proper hand washing is one of the best ways to prevent the spread of germs.
  29. Some bacteria are beneficial. Some cause disease.
  30. What is the causative agent of stomach and duodenal ulcers?
    H. pylori
  31. Choosing to eat yogurt is a healthy ___food.
  32. What kind of bacteria ferment lactose to make lactic acid?
    lactic acid bacteria
  33. Washing out the beaker helps prevent the milk from sticking to the glass.
  34. Which bacteria are in yogurt?
    • Lactobacillus
    • Bifidobacterium
    • Streptococcus
  35. Which term describes the  physical characteristics of a colony?
    colonial morphology
  36. Which tool is used to sterilize?
  37. To make a broth subculture, it's best to directly pour some broth culture into another testtube.
    it has to be a sterile broth culture
  38. Identify the term that describes the growth that settles at the bottom of the test tube.
  39. List the 2 common kinds of bacteria (Genus name) that produce endospores.
    aerobic Bacillus and anaerobic Clostridium.
  40. What causes an endospore to form?
    When adverse environmental conditions arise, these microbes will develop a dormant endospore structure within 6-8 hours
  41. What causes an endospore to germinate?
    When environmental conditions become favorable spores may return to a vegetative state within 1.5 hours.
Card Set:
microbiology lab possibles
2014-09-29 02:29:03

second set
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