You have isolated an unknown bacterium and want to make some morphological observations such as microscopic shape and arrangement. You find some hematoxylin-eosin stain on the bench and apply it to your bacteria. When trying to observe the microbe under the microscope you discover the cells of bacteria are clear. Using your knowledge of stains, explain this observation.
There could have been an error in the process of taking the sample. Such as when the plate was passed through the flame, it was left on too long and it caused the microbe to burn off. Another factor could have been because the more acidic dye does not easily adhere to the cells due to electrostatic repulsion.