What type of colonies do Listeria monocytogenes form when they are grown on bile esculin?
This bacteria is known to come from human bite wounds.
This organism is related to Neisseria and is commonly found in dental plague of humans.
Eikenella is a gram negative bacilli that has what type of smell on an agar?
A bleach type smell
Francisella requires what type of agar for growth?
Blood-cystine-glucose agar with thiamine
L-cysteine, ferric iron, and a range of amino acids are the growth requirements for which species?
What do you plate first with CSF?
SBA and chocolate agar and plate a MAC if gram negative organisms are seen in the gram stain
Francisella has a positive fermentation of which three saccharides?
Glucose, maltose, and mannose
What is a fastidious bacteria?
A microorganism that has special growth requirements
Out of all the fastidious gram positive bacilli, which one does not ferment glucose, lactose, maltose, or sucrose?
Hint: also negative for catalase, indole and urease. Only positive for oxidase
This organism will form a bottle brush growth in a stab culture.
This organism causes the disease Tularemia and can be acquired through handling of infected animal via inhalation, bite, or direct contact with blood.
What does the gram stain of Francisella look like?
Pleomorphic gram negative bacillus with bipolar staining
What do you do when you realize that you cultured Francisella?
Contact the state lab as this organism is a level 3 pathogen.
Name the 6 fastidious fermenters that are clinically significant.
What is the best medium used to grow Gardnerellavaginalis?
Colistin-oxolinic acid blood agar or human bilayer tween (HBT) agar
What does Gardnerella vaginalis require for growth?
What organism do you keep for at least 4 weeks to ensure that the bacteria is not this?
Brucellosis (21-28 days in CO2)
This organism is often found in blood cultures or biopsies of reticuloendothelial (RE) tissue.
What is the new name for Haemophilis?
Haemophilisinfluenza require which two components for growth?
X factor (hemin) and V (NAD or NADP)
Which organism is a gram negative coccobacillis that causes Pertussis or Whooping cough?
What are the motility results of Bordatella?
Out of all the clinical significant fastidious organisms, which one is catalase positive?
In order to be considered free from oropharyngeal contamination, what requirements have to be met?
Less than 10 epithelial cells
greater than 25 neutrophils
Viewed at 10X
On the gram stain, this organism is gram negative rods with one rounded end and one tapered end.
Unlike Eikenella and Kingellakingae which test result differs between these two organisms and C.hominis?
C. hominis is indole +
This organism is catalase positive, oxidase positive, and non-motile. It is commonly associated with animal bites and has a musty odor. It grows on blood and chocolate agar but not MAC. What bacteria is it?
The ALA test speciates between what organisms based on the detection of porphyrin synthesis?
What does ALA disk detect which is excreted by the X-independent Haemophilis strains.
porphobilinogens and porphyrins
Out of the HACEK organisms which is nitrate positive?
Eikenella does not grow well on which two agars?
MAC or EMB
What do 45% of Eikenella do to the agar?
Pit the agar
Which is the least common HACEK group that causes endocarditis?
Which HACEK organism has two different morphological components seen in cultures?
Which organism is the only gram positive that is H2S positive?
Erysipelothrix rhusiopathiae is intrinsically resistant to which antibiotic?
Which is the drug of choice for Erysipelothrix rhusiopathiae?
What is Brucellosis also known as?
What two agars does Francisella grown on?
BCYE and MTM
Does Pasturella Multocida ferment glucose and sucrose?
What is the most common form of infection in Pasturella multocida?
What do you add to the sputum sample with Legionella?
.2N HCL or acid
IS Legionella affect by the chlorine content of water?
Out of the two diseases that Legionella causes, which is non pneumonia causing?
What are the clue cells for an infection with Garderella vaginalis?
Epithelial cells with gram - coccobacilli curved rods
Garderella vaginalis is hippurate what?
Which two agars are commonly used to culture Bordatella?
Bordet-Gengou and Regan Lowe
Why does Bordatella have to be sealed in an airtight bag?
It is a strict aerobe
This HACEK organism tends to have a teardrop shaped colony.
Cardiobacterium hominis does not grow on which media?
Although Cardiobacterium hominis is nitrate negative, what is it able to do with nitrite?
Reduce it to nitrogen gas
Like Eikenella, this organism may also pit the agar.
Kingella kingae is often misidentified as Neisseria or Morexella. What test is used to differentiate them from Kingella?
Kingella is negative and the other two are positive
In order to differentiate between H. aegyptius and H. influenza which test do you preform?
a xylose reaction
What is the major virulence factor for H. influenza?