Microbiology Test 3

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  1. What type of colonies do Listeria monocytogenes form when they are grown on bile esculin?
    Dark colonies
  2. This bacteria is known to come from human bite wounds.
  3. This organism is related to Neisseria and is commonly found in dental plague of humans.
  4. Eikenella is a gram negative bacilli that has what type of smell on an agar?
    A bleach type smell
  5. Francisella requires what type of agar for growth?
    Blood-cystine-glucose agar with thiamine
  6. L-cysteine, ferric iron, and a range of amino acids are the growth requirements for which species?
  7. What do you plate first with CSF?
    SBA and chocolate agar and plate a MAC if gram negative organisms are seen in the gram stain
  8. Francisella has a positive fermentation of which three saccharides?
    Glucose, maltose, and mannose
  9. What is a fastidious bacteria?
    A microorganism that has special growth requirements
  10. Out of all the fastidious gram positive bacilli, which one does not ferment glucose, lactose, maltose, or sucrose?

    Hint: also negative for catalase, indole and urease. Only positive for oxidase
    Eikenella corrodens
  11. This organism will form a bottle brush growth in a stab culture.
    Erysipelothrix rhusiopathiae
  12. This organism causes the disease Tularemia and can be acquired through handling of infected animal via inhalation, bite, or direct contact with blood.
  13. What does the gram stain of Francisella look like?
    Pleomorphic gram negative bacillus with bipolar staining
  14. What do you do when you realize that you cultured Francisella?
    Contact the state lab as this organism is a level 3 pathogen.
  15. Name the 6 fastidious fermenters that are clinically significant.
    • Actinobacillus actinomycetemcomitans
    • Haemophilus aphrophilis
    • Cardiobacterium hominis
    • Kingella kingae
    • Eikenella corrodens
    • Capnophilic Spp.
  16. What is the best medium used to grow Gardnerella vaginalis?
    Colistin-oxolinic acid blood agar or human bilayer tween (HBT) agar
  17. What does Gardnerella vaginalis require for growth?
  18. What organism do you keep for at least 4 weeks to ensure that the bacteria is not this?
    Brucellosis (21-28 days in CO2)
  19. This organism is often found in blood cultures or biopsies of reticuloendothelial (RE) tissue.
    Capnophilic Brucella
  20. What is the new name for Haemophilis?
  21. Haemophilis influenza require which two components for growth?
    X factor (hemin) and V (NAD or NADP)
  22. Which organism is a gram negative coccobacillis that causes Pertussis or Whooping cough?
  23. What are the motility results of Bordatella?
  24. Out of all the clinical significant fastidious  organisms, which one is catalase positive?
    Actinobacillus actinomycetemcomitans
  25. In order to be considered free from oropharyngeal contamination, what requirements have to be met?
    • Less than 10 epithelial cells
    • greater than 25 neutrophils

    Viewed at 10X
  26. On the gram stain, this organism is gram negative rods with one rounded end and one tapered end.
    Cardiobacterium hominis
  27. Unlike Eikenella and Kingella kingae which test result differs between these two organisms and C. hominis?
    C. hominis is indole +
  28. This organism is catalase positive, oxidase positive, and non-motile. It is commonly associated with animal bites and has a musty odor.  It grows on blood and chocolate agar but not MAC. What bacteria is it?
    Pasteurella multocida
  29. The ALA test speciates between what organisms based on the detection of porphyrin synthesis?
    Haemophilis species
  30. What does ALA disk detect which is excreted by the X-independent Haemophilis strains.
    porphobilinogens and porphyrins
  31. Out of the HACEK organisms which is nitrate positive?
    Eikenella corrodens
  32. Eikenella  does not grow well on which two agars?
    MAC or EMB
  33. What do 45% of Eikenella do to the agar?
    Pit the agar
  34. Which is the least common HACEK group that causes endocarditis?
  35. Which HACEK organism has two different morphological components seen in cultures?
    Erysipelothrix rhusiopathiae
  36. Which organism is the only gram positive that is H2S positive?
    Erysipelothrix rhusiopathiae
  37. Erysipelothrix rhusiopathiae is intrinsically resistant to which antibiotic?
  38. Which is the drug of choice for Erysipelothrix rhusiopathiae?
  39. What is Brucellosis also known as?
    Undulant fever
  40. What two agars does Francisella grown on?
    BCYE and MTM
  41. Does Pasturella Multocida ferment glucose and sucrose?
  42. What is the most common form of infection in Pasturella multocida?
  43. What do you add to the sputum sample with Legionella?
    .2N HCL or acid
  44. IS Legionella affect by the chlorine content of water?
  45. Out of the two diseases that Legionella causes, which is non pneumonia causing?
    Pontiac Fever
  46. What are the clue cells for an infection with Garderella vaginalis?
    Epithelial cells with gram - coccobacilli curved rods
  47. Garderella vaginalis is hippurate what?
  48. Which two agars are commonly used to culture Bordatella?
    Bordet-Gengou and Regan Lowe
  49. Why does Bordatella have to be sealed in an airtight bag?
    It is a strict aerobe
  50. This HACEK organism tends to have a teardrop shaped colony.
    Cardiobacterium hominis
  51. Cardiobacterium hominis does not grow on which media?
  52. Although Cardiobacterium hominis is nitrate negative, what is it able to do with nitrite?
    Reduce it to nitrogen gas
  53. Like Eikenella, this organism may also pit the agar.
    Cardiobacterium hominis
  54. Kingella kingae is often misidentified as Neisseria or Morexella. What test is used to differentiate them from Kingella?

    Kingella is negative and the other two are positive
  55. In order to differentiate between H. aegyptius and H. influenza which test do you preform?
    a xylose reaction
  56. What is the major virulence factor for H. influenza?
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Microbiology Test 3
2014-09-24 01:53:46

Micro test 3
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