Aside from the moist chamber technique, give other methods by which once can isolate molds.
Direct Transfer: This isolation method would be useful if the growth of the molds would be visualized. The object that would be the place of the growth of molds would be placed under a dissecting microscope and an inoculating needle is heated, cooled then used to get some spores into a sterile plate of culture medium. Direct Plating: This method is used to rapidly spread molds and fungi with the use of a nutrient agar medium. Pieces of substance where the molds will grow are placed on the surface of the agar. This method is usually used for soil studies that only requires a pinch of soil that is dispersed over the surface of the agar. Dilution Plating: In this technique 1 gram (dry weight) of the material to be studied is ground up (if necessary) and dispersed in 9 ml of sterile water. One millilitre of this solution is transferred to a second tube containing 9 ml of sterile water, resulting in a 0.01 dilution of the spore mass in the original material. The process is repeated to yield dilutions of 0.001, 0.0001, and 0.00001 or even further if necessary. A 1-ml portion from each dilution is pipetted to a separate Petri dish, and cooled, melted agar medium poured over it. The plate should be moved gently on the table in a figure-of-eight motion to effect proper dispersion. Alternatively, the solution can be put on the surface of solidified medium and spread evenly throughout.