How does phosphorylation regulate protein function? What enzyme is used to add the phosphate group to the protein? What type of bond is used? It attracts a cluster of what and what charge do they have? How does that affect the binding of the ligand to the protein surface?
How does phosphorylation stimulate a protein's activity into larger complexes?
Two ways that phosphorylation inhibit or stimulate a reaction.
How is the phosphate group introduced in the first place?
Kinsase is used to add the phosphate group to the protein. The phosphate group covalently attaches to one or more of the protein's amino acid side chains causing a major conformational change in the protein by attracting a cluster of positively charged amino acids which in turn affects the binding of ligands elsewhere on the protein surface, altering its activity.
The cluster of positively charged amino acids causes conformational change in the protein structure causing the ligand binding altered on the protein surface.
By creating docking sites where other proteins can bind.
By changing the protein's conformation and serving as docking sites for other proteins to attach.