biochem 007 mechanisms and inhibitors (optimal pH inhibition competitive inhibition uncompetitive

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  1. what common strategies do enzymes employ to catalyze specific reactions?
    • 1. Covalent catalysis
    • 2. general acid base catalysis
    • 3. Metal ion catalysis
    • 4. Catalysis by approximation and orientation
  2. what is covalent catalysis
    in covalent catalysis, the active site contains a reactive group, usually a powerful nucleophile that becomes temporarily covalently modified in the course of catalysis. The proteolytic enyme chymotrypsin provides an excellent example of this mechanism
  3. what is general acid-base catalysis
    in general acid-base catalysis, a molecule other than water plays the role of a proton donor or acceptor. Chymotrypsin uses a histidine residue as a base catalyst to enhance the nucleophilic power of the serine
  4. what is metal ion catalysis
    metal ions can function catalytically in several ways. For instance, a metal ion may serve as an electrophilic catalyst, stabilizing a negative charge on a reaction intermediate. Alternatively, a metal ion may generate a nucleophile by increasing hte acidity of a nearby moleculem such s water. Finally, a metal ion may bind to the substrate increasing the number of interactions with the enzyme and thus the binding energy. Metal ions are required cofactors for many of the nezymes that we will encounter in our study of biochemistry
  5. what is catalysis by approximation and orientation?
    many reactions include two distinct substrates. in such cases, the reaction rate may be considerably enhanced by bringing the two substrates into proximity and in the proper oritentation on a signle binding surface of an enzyme
  6. what are the three types of reversible inhibition?
    competitive, non-competitive, and uncompetitive
  7. How does suicide inhibition work?
    a suicide inhibitor is a chemically modified substrate. The inhibitor binds to the enzyme as a substrate and is initially processed by the normal catalytic mechanism. The mechanism of catalysis then generates a chemically reactive intermediate that inactivates the enzyme through covalent modification.
  8. What does penicillin and aspirin have in common?
    they are both irreversible inhibitors
Card Set
biochem 007 mechanisms and inhibitors (optimal pH inhibition competitive inhibition uncompetitive
biochem 007 mechanisms and inhibitors (optimal pH, inhibition, competitive inhibition, uncompetitive inhibition, antibiotics, chymotrypsin, proteases) #12
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