When does proofreading happen?
How many domains does DNA Polymerase have, and what are they?
What happens when an incorrect nucleotide is added and does DNA Polymerase continues on?
How is the cleaving of the incorrect nucleotide from the strand done?
At the same time as DNA synthesis occurs.
Polymerization domain and Proofreading domain.
DNA polymerase cleaves the incorrect nucleotide from the strand and replaces it with the correct one before continuing.
The new (synthesized) DNA strand transiently unpairs from the template strand and the 3' end moves into the error-correcting catalytic site (E) to be removed.