bio of nucleus 10/30

The flashcards below were created by user xijunzhu on FreezingBlue Flashcards.

  1. what is the primer for viral RNA genome?
    tRNA
  2. how to synthesize cDNA
    need to have primer oligo-dT (short sequence of deoxy-thymine nucleotides), add to poly A tail
  3. how to make cDNA?
    • isolated mRNA from cytoplasm and add reverse transcriptase, synthesize cDNA strand and remove RNA.
    • synthesize cDNA with fluorescent NT (A or T) tag, when it is attached to mRNA from sample (it can fluoresce under microarray)
  4. what is dideoxy DNA sequencing?
    prevents extension at 3' end during replication
Author
ID
287888
Card Set
bio of nucleus 10/30
Description
bio
Updated
Show Answers