bio of nucleus 10/30

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Author:
xijunzhu
ID:
287888
Filename:
bio of nucleus 10/30
Updated:
2014-11-02 17:41:37
Tags:
bio
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Description:
bio
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The flashcards below were created by user xijunzhu on FreezingBlue Flashcards. What would you like to do?


  1. what is the primer for viral RNA genome?
    tRNA
  2. how to synthesize cDNA
    need to have primer oligo-dT (short sequence of deoxy-thymine nucleotides), add to poly A tail
  3. how to make cDNA?
    • isolated mRNA from cytoplasm and add reverse transcriptase, synthesize cDNA strand and remove RNA.
    • synthesize cDNA with fluorescent NT (A or T) tag, when it is attached to mRNA from sample (it can fluoresce under microarray)
  4. what is dideoxy DNA sequencing?
    prevents extension at 3' end during replication

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