ch 12 transcription genetics FINAL

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doncheto
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289511
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ch 12 transcription genetics FINAL
Updated:
2014-11-18 17:45:14
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genetics transcription
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  1. prok. Trxn
    polycistronic
  2. Polycistronic
  3. Promoter Sequence
    • "consensus" sequence
    •   - 2 regions appear important
    •       -10, -35
    • Faster rate of trxn if regions are similar
  4. -10 region
    Pribnow box
  5. RNA Polymerase (E-coli)
    • 5 main sub units
    •  4 catalytic
    •  1 regulatory
    • Alpha (x2) assembly of care enzyme
    • Beta Beta'(Regulatory)- binding to DNA
    •   - Help w/ RNA synthesis 
    • Sigma - binds to -10, -35 promoter
    •    E-coli has several Sigma factors
  6. E-coli has several Sigma factors
    Sigma70 : used in majority of genes
  7. Transcription bubble - elongation
  8. Termination
    • 1. Rho - independent "instrinsic"
    •  - more simplistic
    • terminator sequences: ~40 bp  GC rich followed by long run of A's
    • 2. Rho-dependent
    •    40-60 bp rich in C, poor in G, and rho-utilization site "Rut"
  9. Eukaryotes
    • Initiation: in general more complicated
    •     -diff cellular compartments
    •     RNA processing (gene regulation)
    •    DNA is organized into chromatin
    •    size of genome
    •       -genes are farther apart 
    •            -How does RNA Pol find start codon?`
  10. *Multiple RNA Polymerases
    • RNA Pol  I: ALL Rrna (NOT 5S)
    •                II: all mRNA, some sn RNA
    •                III: 5s, tRNA's
  11. Requires assembly of General Transcription
    • Factors GTF's   
    •  -necessary for function of RNA Pol II
  12. TFIIF
    Hangs onto RNA Pol II
  13. TAF
    TBP-Associates Factors
  14. TFIIH
    TFIIH: Acts as a helicase, opens DNA, creates txrn bubble.
  15. TFIID
    1. Acts as a helicase, opens DNA, creates trxn bubble

    • 2. Also PO4 the carboxyl terminal domain (CTD) on RNA Pol II,
    •    -causes contact with  TFII B,H,E breaks
  16. Elongates.
    nothing new
  17. Termination
    • : RNA Pol transcribes past poly A
    • 2 models
    • 1. "Allosteric model"
    •   -elongation factors release or
    •   -termination factors bind
    • 2. "Torpedo Model"
    •   -exonuclease binds to 5' end of RNA
    •   -degrades it moving towards RNA Pol II
    •   -hits RNA Pol and Knocks it off

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