When do you use CaCl in transformation in transformation and why?
If a bacterium readily takes foreign DNA, it is a competent cell. This is not occurring naturally but can be chemically induced with CaCl.
Bacterial membrane has negatively charged phosphates. The positive charged calcium ions stabilize the negatively charged phosphates, and the low temperature makes it rigid, making the entire cell membrane stablized. Now that it's stablized, the plasmid DNA can be introduced.