Glycolytic Pathway

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  1. Glucose-----> Glucose 6-phosphate

    ATP used--> ADP
  2. Glucose 6-phosphate---> Fructose 6-phosphate
    phosphoglucose isomerase
  3. Fructose 6-phosphate---> fructose 1,6-bisphosphate

    ATP used and converted to ADP
  4. Fructose 1,6-bisphosphate--->??
    aldose---> Dihydroxyacetone phosphate and glyceraldehyde 3-phosphate
  5. Dihydroxyactone phosphate--> glyceraldehyde 3-phosphate
    triose phosphate isomerase
  6. Glyceraldehyde 3-phosphate----> 1,3 bisphoglycerate
    glyceraldehyde-3-phosphate dehydrogenase

    NAD+ to NADH
  7. 1,3-bisphosphoglycerate--> 3-phosphoglycerate
    phosphoglycerate kinase

    ADP gets converted to ATP
  8. 3-phosphoglycerate--> 2-phosphoglycerate
    phosphoglycerate mutase
  9. 2- phosphoglycerate--> phosphoenolpyruvate
    enolase (loss of H2O)
  10. Phosphoenolpyruvate--> pyruvate
    pyruvate kinase 

    ADP--> ATP
  11. Where is ATP consumed?
    in the step catalyzed by hexokinase and the step catalyzed by phosphofructokinase
  12. Where is ATP made?
    in the step catalyzed by phosphoglycerate kinase and pyruvate kinase
  13. Where is NADH produced
    in the step catalyzed by glyceraldehyde 3-phosphate dehydrogenase
  14. How many times does the second stage happen?
  15. What are the intermediates in order in stage one of glycolysis?
    • glucose
    • glucose 6-phosphate
    • fructose 6-phosphate
    • fructse 1,6-bisphosphate
    • dihydroxyacetone phosphate and glyceraldehyde 3-phosphate
  16. What are the intermediates in order in stage two of glycolysis?
    Glyceraldehyde 3-phosphate




  17. What is the catalytic mechanism of G 3P dehydrogenase?
    the reaction proceeds through a thioester intermediate, allowing the oxidation of glyceraldehyde to be coupled to the phosphorylation of 3-phosphoglycerate

    • 1) Cysteine reacts with the aldehyde group of the substrate, forming the hemithioacetal
    • 2) oxidation with transfer of H to NAD+, forin a thioester (this step is facilitated by the transfer of a proton to histidine
    • 3) the reduced NADH is exchanged for an NAD+ molecule
    • 4) Orthophosphate attacks the thioester, forming the product 1,3-BPG
  18. What is the mechanism of triose phosphate isomerase?
    Glutamate acts as a base by abstracting a proton from carbon 1

    histidine acts as an acid, donating a proton to the oxygen atom bonded to carbon 2, forming the enediol intermediate

    Glutamic acid now acts as an acid, donating a proton C2 while histidine remvoes a proton from the OH group of C1

    the product forms and glutamate and histidine are back to ionized forms
Card Set:
Glycolytic Pathway
2014-11-23 01:09:46
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