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Glucose-----> Glucose 6-phosphate
ATP used--> ADP
Glucose 6-phosphate---> Fructose 6-phosphate
Fructose 6-phosphate---> fructose 1,6-bisphosphate
ATP used and converted to ADP
aldose---> Dihydroxyacetone phosphate and glyceraldehyde 3-phosphate
Dihydroxyactone phosphate--> glyceraldehyde 3-phosphate
triose phosphate isomerase
Glyceraldehyde 3-phosphate----> 1,3 bisphoglycerate
NAD+ to NADH
ADP gets converted to ATP
2- phosphoglycerate--> phosphoenolpyruvate
enolase (loss of H2O)
Where is ATP consumed?
in the step catalyzed by hexokinase and the step catalyzed by phosphofructokinase
Where is ATP made?
in the step catalyzed by phosphoglycerate kinase and pyruvate kinase
Where is NADH produced
in the step catalyzed by glyceraldehyde 3-phosphate dehydrogenase
How many times does the second stage happen?
What are the intermediates in order in stage one of glycolysis?
- glucose 6-phosphate
- fructose 6-phosphate
- fructse 1,6-bisphosphate
- dihydroxyacetone phosphate and glyceraldehyde 3-phosphate
What are the intermediates in order in stage two of glycolysis?
What is the catalytic mechanism of G 3P dehydrogenase?
the reaction proceeds through a thioester intermediate, allowing the oxidation of glyceraldehyde to be coupled to the phosphorylation of 3-phosphoglycerate
- 1) Cysteine reacts with the aldehyde group of the substrate, forming the hemithioacetal
- 2) oxidation with transfer of H to NAD+, forin a thioester (this step is facilitated by the transfer of a proton to histidine
- 3) the reduced NADH is exchanged for an NAD+ molecule
- 4) Orthophosphate attacks the thioester, forming the product 1,3-BPG
What is the mechanism of triose phosphate isomerase?
Glutamate acts as a base by abstracting a proton from carbon 1
histidine acts as an acid, donating a proton to the oxygen atom bonded to carbon 2, forming the enediol intermediate
Glutamic acid now acts as an acid, donating a proton C2 while histidine remvoes a proton from the OH group of C1
the product forms and glutamate and histidine are back to ionized forms
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