What is the mechanism of triose phosphate isomerase?
Glutamate acts as a base by abstracting a proton from carbon 1
histidine acts as an acid, donating a proton to the oxygen atom bonded to carbon 2, forming the enediol intermediate
Glutamic acid now acts as an acid, donating a proton C2 while histidine remvoes a proton from the OH group of C1
the product forms and glutamate and histidine are back to ionized forms