Proteins Biopharmaceuticals

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  1. level of structure of protein that is a sequence of a chain of amino acids
    primary protein structure
  2. a level of structure of protein that occurs when the sequence of amino acids are linked by hydrogen bonds
    secondary protein structure
  3. a level of structure of protein that occurs when certain attractions are preserved between alpha helices and pleated sheets
    tertiary protein structure
  4. a level of structure of proteins that occurs when there are more than one amino acid chains attached together
    quaternary protein structure
  5. what are the 3 sources of protein biopharmaceuticals?
    • microorganisms (ecoli, yeast, fungal)
    • animal cell culture systems
    • transgenic animals/plants
  6. what are the 3 parts of upstream processing for biopharmaceutical production?
    • 1. get cells from cell bank
    • 2. propagation of working bank cells, generating starter cultures
    • 3. production scale cell culture
  7. what are the 6 parts of downstream processing for biopharmaceutical production?
    • 1. harvest cells to recover crude product
    • 2. concentration
    • 3. main purification
    • 4. final product formulation
    • 5. product filling, freezing, drying, and sealing
    • 6. labeling and packaging
  8. region of the antigen recognized by an antibody
  9. antibodies secreted from a single B cell, have identical paratopes
    monoclonal antibodies
  10. any substance capable of eliciting an adaptive immune response
  11. immunoglobulin secreted by B cells
  12. what is the mechanism of action of antibody?
    • 1. blocikg /neutralizing action of molecular targets
    • 2. antibody-dependent celuular cytotoxicity (mark target cells or immune cell to attack)
    • 3. signal molecules
    • 4. delivery agents
  13. what is a hybridomas technology?
    inject mice with antigen and then fuse immune cells with tumor cells to produce hybridomas. The hybridomas are mass produced and then monoclonal antibodies are isolated.

Card Set Information

Proteins Biopharmaceuticals
2014-12-15 00:35:49

development processes, upstream and downstream processing, product analysis
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