Biochem exam 3 study
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What level of structure refers to the three-dimensional arrangement of all the atoms in one polypeptide chain?
Tertiary (multiple chains would be quad)
Which of the following are reducing sugars?
Basically sucrose is the only non reducing sugar, it has a 5 membered ring. All other are reducing
The titration curve for D would show...
3 plateaus, the acidic ones (D and E) and the basic lysine (K), would show 3, assume 2 for others?
Archaebacteria have modified their lipids to accommodate their environment through
Some proteins are anchored to the membrane by being covalently attached to a ------ group by a thioester linkage to a specific cysteine residue.
A buffer solution at pH 5.0 has a ratio of [HA]/[A-]=100. What is the pKa of the acid.
Refer to the following information, what is the predominant form of the amino acid abbreviated Y at pH1?
positive, any amino acid would be. If neutral know that (E and D) are acidic, and (K,H, and N) are basic
What groups are present in M at a pH of 11?
COO- nad NH2, think its more basic so less hydrogens
The peptide bond
- -is usually trans (more stable)
- -does not have free rotation
- -the six atoms are planar
A-helices are different from B-sheets because
the hydrogen bones are parallel to axis in a helices and the hydrogen bonds are perpendicular in B sheets
When the first oxygen binds to FE in heme of Hb, the Fe is drawn into the plane of the the porphyrin ring, True or false?
Which of the followings about Fe in heme is correct?
the distal histidine helps to minimize oxidation
The second law of thermodynamics states
the total entropy of a system and its surroundings always increases
The oligonucleotide pAGGTCCATTGAA is best described as
A DNA oligonucleotide with a 5' phosphate
The fetal hemglobin has higher affinity to O2 than maternal because
fetus hemoglobin has lower affinity to BPG
Anaerobic coniditons due to exercise or due to tumor growth activate the transcription factor HIF-1 which
- has multiple subunits
- performs "induced fit" when binding to glu
increases in F2,6BP lead to activation of PFK-1 is a type of
The following reaction (4h 4 e thing) usually demonstates
- -reductive synthesis of fatty acid
- -electrons usually are provided by NADPH
has a low Km and performs basal glucose transportation
the rossman fold is
an NAD+ binding motif and is found in LDH and ADH
The activity of the enzyme phosphofrutokinase is
stimulated by fructose 2,6-bisphophate
Inhibition of PFK would also likely cause what to happen in the cell?
inhibition of hexokinase
making some bigger (even when using water) is a type of...
the conversion of pyruvate to lactate
- -is the process that results in sore muscles after exercise
- -allows for recycling of NAD+
cAMP activates PKA by...
attaching to the regulator subunit of PKA
Which of the following is an example of a compound that has lower phosphate transfer potential compared to ATP
NADH is produced at which step of glycolysis?
glyceraldehyde 3 phosphate dehydrogenase catalyzed reaction
The last ATP production in glycolysis is catalyzed by
Which of the following has the lowest transfer free energy (tranfer from inner membane to extracellular environments?
The amino acid histidine plays an important roll physiologicall because
it serves as a proton shuttle
If the pattern of H-bonding is always the C=O binds to the N-H of residue i+4 within the same peptide chain, the structure is called..
cellulose vs glycogen?
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